文献类型：期刊文献

中文题名：反相/强阳离子交换色谱混合固定相的分离选择性研究

英文题名：Study on the Separation Selectivity of Reverse Phase/Strong Ion Exchange Chromatographic Mixed Stationary Phase

作者：卢国庆[1];张凌怡[1];张维冰[1]

机构：[1]华东理工大学化学与分子工程学院上海市功能性材料化学重点实验室,上海200237

年份：2025

卷号：44

期号：9

起止页码：1924

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：;北大核心:【北大核心2023】；

基金：中央高校基本科研业务费专项资金资助项目(NJKJ01251717)。

语种：中文

中文关键词：混合模式色谱;混合床固定相;死时间;保留因子

外文关键词：mixed mode chromatography;mixed bed stationary phase;dead time;retention factor

摘要：该文对混合固定相装填色谱柱中溶质的色谱行为进行系统分析,从理论上得到了反映溶质在不同填料比例色谱柱上保留因子与固定相组成关系的表达式。进一步将相同尺寸和形态基质制备的反相和强阳离子交换固定相混合,装填成6根不同比例的混合床色谱柱。以苯系物、氨基酸、生物碱类样品为模型,实验测得保留因子变化趋势与理论表达式一致。以川贝为样品考察其在混合床色谱柱中的分离行为,结果表明不同配比的固定相对不同结构的溶质提供的保留行为有显著差异,其趋势与理论预测一致。混合固定相充分发挥不同分离模式的特点,并可通过其原固定相的色谱保留行为预测新色谱柱的分离行为,为色谱柱及分离模式设计提供了新思路,具有广阔的应用前景。
This article systematically analyzed the chromatographic behavior of solutes in mixed mode columns packed with C18 and SCX stationary phases in different proportions,obtaining theoretical expression of relationship between the retention factor of solutes on mixed mode chromatographic columns and packing ratios of the two stationary phases.Six mixed mode chromatography columns were prepared by mixing C18 stationary phase and strong cation exchange stationary phase prepared by the same silicon matrix with exact size and morphology in different packing ratios.The dead time of the mixed bed chromatography columns was measured by sodium nitrate marker method and Peterson-Hirsch method,which were used to predict retention factor trends with benzene derivatives,amino acids,alkaloids as model samples according to the theoretical expression.The predicted results were compared with the experimental data,showing the same trend.Sichuan Fritillaria sample was used as real sample to evaluate the performance of the mixed mode columns.The mixed mode columns packed with two stationary phases in different ratios provide significantly different retention behavior for solutes with different structures,and the variation trends were consistent with theoretical predictions.Obviously,the mixed bed column can fully utilize the characteristics of different separation modes,and the selectivity can be easily controlled by changing the packing ratio.The separation behavior of solute on the mixed bed column can be predicted based on its retention behavior on two original stationary phases,which provides a new idea for the design of chromatographic columns and separation modes,showing broad application prospects.