文献类型：期刊文献

英文题名：Oxytetracycline biosynthesis improvement in Streptomyces rimosus following duplication of minimal PKS genes

作者：Yu, Lan[1,2];Cao, Nan[1];Wang, Long[1];Xiao, Ciying[1];Guo, Meijin[1];Chu, Ju[1];Zhuang, Yingping[1];Zhang, Siliang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Huzhou Teachers Coll, Sch Life & Sci, Huzhou 313000, Zhejiang, Peoples R China

年份：2012

卷号：50

期号：6-7

起止页码：318

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000303955700008)】；

基金：This work was supported by grants from the National High Technology and Development Program of China (2012AA021201), the Outstanding Youth Talent Plan of East China University of Science and Technology (ECUST) and the Open Project Program for State Key Laboratory of Bioreactor Engineering (No. 2060204).

语种：英文

外文关键词：Minimal PKS genes; Overexpression; Morphology; Oxytetracycline

摘要：Oxytetracycline (OTC) is a widely used antibiotic, which is commercially produced by Streptomyces rimosus. The type II minimal polyketide synthases (minimal PKS) genes of the oxytetracycline biosynthesis cluster in S. rimosus, consisting of oxyA, oxyB and oxyC, are involved in catalyzing 19-C chain building by the condensation of eight malonyl-CoA groups to form the starting polyketide. This study aimed to investigate the effects of overexpression of the minimal PKS gene in a model S. rimosus strain (M4018) and in an industrial overproducer (SR16) by introduction of a second copy of the gene into the chromosome. Increased levels of oxyA, oxyB and oxyC gene transcription were monitored using reverse transcription quantitative real-time PCR. Overexpression of the minimal PKS gene elicited retardation of cell growth and a significant improvement in OTC production in corresponding mutants (approximately 51.2% and 32.9% in M4018 and SR16 mutants respectively). These data indicate that the minimal PKS plays an important role in carbon flux redirection from cell growth pathways to OTC biosynthesis pathways. (C) 2012 Elsevier Inc. All rights reserved.