文献类型：期刊文献

英文题名：Effects of antibiotic-induced resistance on the growth, survival ability and virulence of Salmonella enterica

作者：Hong, Yi[1];Wu, Yufan[2];Xie, Yani[1];Ben, Leijie[1];Bu, Xiangfeng[1];Pan, Xinye[1];Shao, Jingdong[3];Dong, Qingli[1];Qin, Xiaojie[1];Wang, Xiang[1,4]

机构：[1]Univ Shanghai Sci & Technol, Sch Hlth Sci & Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Ctr Anal & Test, Sch Chem & Mol Engn, Shanghai, Peoples R China;[3]Technol Ctr Zhangjiagang Customs, Suzhou, Peoples R China;[4]Univ Shanghai Sci & Technol, Sch Hlth Sci & Engn, Jungong Rd 516, Shanghai 200093, Peoples R China

年份：2023

卷号：115

外文期刊名：FOOD MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001029551700001)】；

基金：This Shanghai Agriculture Applied Technology Development Pro- gram, China (Grant No. 2022-02-08-00-12-F01144) and the National Natural Science Foundation of China (Grant No. 31801455) .

语种：英文

外文关键词：Antibiotic resistance; Foodborne pathogen; Biological characteristics; Potential pathogenicity; Risk assessment

摘要：Salmonella enterica is an important foodborne pathogen that constitutes a major health hazard. The emergence and aggravation of antibiotic-resistant Salmonella has drawn attention widely around the world. Conducting a risk assessment of antibiotic-resistant foodborne pathogens throughout the food chain is a pressing requirement for ensuring food safety. The growth, survival capability, and virulence of antibiotic-resistant Salmonella represent crucial biological characteristics that play an important role in microbial risk assessment. In this study, eight antibiotic-sensitive S. enterica strains were induced by Ampicillin (Amp) and Ciprofloxacin (CIP), respectively, and AMP-resistant and CIP-resistant mutants were obtained. The growth characteristics under different temperatures (25, 30, 35 degrees C), viability after exposure to heat (55, 57.5, 60 degrees C) and acid (HCl, pH = 3.0), the virulence potential (adhesion and invasion to Caco-2 cells, biofilm formation and motility) and the lethality in a model species (Galleria mellonella) were evaluated and compared for S. enterica strains before and after antibiotic exposure. The induction by AMP and CIP are likely to promote cross-antibiotic resistance to their antibiotic classes, & beta;-lactams and quinolones, as well as some compound antibiotics. It was observed that generally the antibiotic-induction-resistant strains showed decreased growth ability and lower heat resistance, although the differences were not significant at all the conditions tested. The AMP-resistant strains were significantly less acid resistance than the sensitive and the CIP-resistant ones, while exhibiting increased biofilm formation ability. In general, the antibiotic-induced resistance did not significantly affect the motility, adherence, or invasion ability of Caco-2 cells. However, CIP-resistant strains displayed lower lethality in G. mellonella infection, whereas AMPresistant strains did not, and even two strains improved lethality. The study of the biological characteristics of antibiotic-resistant S. enterica is essential in better understanding the microbial risks to both the food chain and human health, thereby facilitating a more accurate risk assessment.