文献类型：期刊文献

英文题名：Umami polypeptide detection system targeting the human T1R1 receptor and its taste-presenting mechanism

作者：Zhang, Chuanxi[1,2,4];Miao, Yulu[1];Feng, Yinghui[1];Wang, Jiawei[1,5];Tian, Zhuoli[1];Dong, Juan[6];Gao, Bei[2];Zhang, Lujia[1,3]

机构：[1]East China Normal Univ, Shanghai Engn Res Ctr Mol Therapeut & New Drug De, Sch Chem & Mol Engn, Shanghai 200062, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[3]NYU Shanghai, NYU ECNU Ctr Computat Chem, Shanghai 200062, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Dept Micro Nano Elect, Shanghai 200240, Peoples R China;[5]Univ Shanghai Sci & Tecchnol, Sch Hlth Sci & Engn, Shanghai 200093, Peoples R China;[6]Shihezi Univ, Sch Food Sci & Technol, Shihezi 832000, Peoples R China

年份：2022

卷号：287

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20222712329106);WOS:【SCI-EXPANDED(收录号:WOS:000861551000002)】；

基金：This work was supported by the National Key R&D Program of China (grant no. 2020YFA0908400), National Natural Science Foundation of China (grant nos. U1805235, 31772007), Natural Science Foundation of Shanghai (grant nos. 21ZR1416500). Thanks to the biacore platform of Shanghai Jiao Tong University instrumental analysis center for providing technical guidance.

语种：英文

外文关键词：Umami petides; hT1R1 receptor; Interaction; Detection system

摘要：Umami is one of five basic tastes, the elucidation of its mechanism by the study of the interaction between umami polypeptides and hT1R1 umami receptors is of great significance. However, research on umami peptides targeting human T1R1 receptors is lacking, and the molecular mechanism remains elusive. Here, we successfully established a system to detect umami peptides targeting human T1R1 receptors by fluorescence spectroscopy, Surface Plasmon Resonance (SPR) and computational simulation. The sensory evaluation, calculated Kd value, and experimental affinity results between the four selected umami peptides (GRVSNCAA, KGDEESLA, KGGGGP, and TGDPEK) and glutamate were tested using this system, and all matched well. The maximum Ka value of GRVSNCAA was 479.55 M-1, and the minimum affinity of TGDPEK was 2.67 M-1. Computational simulations showed that the different peptide binding sites in the hT1R1 binding pocket occupied due to conformational changes are important factors for different taste thresholds, and that peptide hydrophobicity plays an important role in regulating affinity. Thus, our study enables rapid screening of high-intensity umami peptides and the development of T1R1 receptor-based umami detection sensors.