文献类型：期刊文献

英文题名：YebC controls virulence by activating T3SS gene expression in the pathogen Edwardsiella piscicida

作者：Wei, Lifan[1];Wu, Yanyan[1];Qiao, Haoxian[1];Xu, Wensheng[2];Zhang, Yuanxing[1,3,4];Liu, Xiaohong[1,3,4];Wang, Qiyao[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Beijing Agr Univ, Fac Food Sci & Engn, Lab Agr Prod Detect & Control Spoilage Organisms, Beijing 102206, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg T, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2018

卷号：365

期号：14

外文期刊名：FEMS MICROBIOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000441114900010)】；

基金：The study was supported by National Natural Science Foundation of China, grant nos. 31 602 200 (XHL), and 31 430 090 (YXZ), the Ministry of Agriculture of China, grant no. CARS-47-G17 (QYW), Shanghai Pujiang Program, grant no. 16PJD018 (QYW), and the Science and Technology Commission of Shandong and Shanghai Municipality (grant nos. 2017CXGC0103 and 17 391 902 000) (QYW). WSX was supported by Beijing Municipal Commission of Education (grant no. KM201310020010).

语种：英文

外文关键词：Edwardsiella piscicida; YebC; T3SS; QS; Tn-seq

摘要：Edwardsiella piscicida is an infectious Gram-negative bacterium that causes great losses to the aquaculture industry worldwide. Based on pattern analysis of conditional essentiality (PACE), a new method for transposon insertion sequencing (Tn-seq) data analysis, we investigated the genome-wide genetic requirements during the dynamic process of infection and colonization in turbot in this study. As a result, disruption of ETAE_1437 was discovered to lead to substantially reduced colonization, which was similar to the in vivo dynamic patterns of the mutants of T3SS or T6SS. Bioinformatics analysis indicated that ETAE_1437 is a YebC/PmpR family regulator. Moreover, we found that ETAE_1437 not only regulated quorum sensing by directly binding to the edwR promoter region but also activated T3SS expression by directly binding to the promoter region of the T3SS gene ETAS 0873. In addition, ETAE_1437 mutants exhibited substantial colonization defects and significantly decreased virulence in turbot. Overall, this study identified ETAE_1437 as a novel virulence regulator in E. piscicida and enriched our understanding of the pathogenesis of E. piscicida in fish. We thus reannotated ETAE_1437 as YebC.