文献类型：期刊文献

中文题名：紫娟茶提取物对血管紧张素转换酶、α-淀粉酶和胰脂肪酶的体外抑制作用

英文题名：Inhibitory effects of Zijuan tea(Camellia sinensis var.kitamura)extracts on angiotensin converting enzyme,α-amylase and pancreatic lipase in vitro

作者：赵瑜[1];周家春[1];张靖伟[1];张宛昕[1];赵黎明[1];蒋丽华[1]

机构：[1]华东理工大学,生物反应器工程国家重点实验室,发酵工业分离提取技术研发中心,上海200237

年份：2017

卷号：38

期号：19

起止页码：11

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

语种：中文

中文关键词：紫娟茶提取物;总酚;花色苷;茶多酚;血管紧张素转换酶;α-淀粉酶;胰脂肪酶

外文关键词：Zijuan tea extracts;total phenols;anthocyanin;tea polypheno];angiotensin converting enzyme;α-amylase;pancreatic lipase

摘要：目的:探究紫娟茶提取物是否具有一定潜在的降血压、降血糖和减肥功效,并分析紫娟茶提取物中茶多酚和花色苷在其中起到的作用。方法:对紫娟茶进行提取,将紫娟茶粗提物(ZTE_1)再分离为花色苷的粗提物(ZTE_2)与茶多酚的粗提物(ZTE_3),将ZTE_2进一步纯化分离出两种纯化花色苷——飞燕草-3-O-β-D-(6-(E)-对香豆酸)吡喃半乳糖苷(delphinidin-3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside,AN_3)和矢车菊-3-O-β-D-(6-(E)-对香豆酸)吡喃半乳糖苷(cyanidin-3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside,AN_4);分别评价ZTE_1、ZTE_2、ZTE_3、AN_3和AN_4对血管紧张素转换酶(Angiotensin converting enzyme,ACE)、α-淀粉酶和胰脂肪酶的体外抑制作用,并与表没食子儿茶素没食子酸酯(Epigallocatechin gallate,EGCG)进行比较,评价了AN3和AN4对三种酶的抑制类型。结果:ZTE1、ZTE2、ZTE3、AN3和AN4对三种酶均有一定的抑制作用,粗提取物中,ZTE3对三种酶、ZTE1对α-淀粉酶和胰脂肪酶、ZTE2对α-淀粉酶的抑制超过或相当于EGCG的效果,其中ZTE3对ACE、ZTE2对α-淀粉酶、ZTE1对胰脂肪酶的抑制效果最强;纯化花色苷中,AN3对α-淀粉酶的抑制效果较好,与EGCG无显著性差异,AN3对三种酶的抑制效果均优于AN4;此外,AN3和AN4对三种酶均为非竞争性抑制,抑制常数Ki分别为:0.33、0.37 mmol/L;0.39、0.41 mg/m L;0.22、0.78 mg/m L。结论:紫娟茶对高血压、高血糖和肥胖症有一定的潜在功效。
Objective:To explore whether the extracts of Zijuan tea has the potential to anti-hypertensive,anti-hyperglycemic and anti-obesity.Methods:The crude polyphenol fraction(defined as ZTE1)was extracted from Zijuan tea.ZTE1 was then separated to the precipitated(defined as ZTE2)and the soluble(defined as ZTE3)with ethyl acetate.Subsequently,delphinidin-3-O-B-D-(6-(E)-P-coumaroyl)galactopyranoside(AN3)and cyanidin-3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside(AN4)were purified from ZTE2.The inhibitory activities of the three crude fractions and two anthocyanins towards angiotensin converting enzyme(ACE),α-amylase and pancreatic lipase were evaluated comparing with epigalloeatechin gallate(EGCG).The inhibitory type of AN3 and AN4 towards ACE,a-amylase and pancreatic lipase was also explored.Results:All the Zijuan tea extracts had inhibitory activities towards the three enzymes.The inhibitory activities of ZTE3 towards the three enzymes,ZTE1 towards a-amylase and pancreatic lipase,ZTE2 towards a-amylase were stronger than EGCG.Furthermore,ZTE3 had the highest inhibitory acitivity against ACE,whereas ZTE2 and ZTE3 had the highest inhibitory acitivity against a-amylase and pancreatic lipase,respectively.And the inhibitory activity of AN3 towards a-amylase was not significantly different to EGCG.The inhibitory activity of AN3 was higher than AN4 towards all the three enzymes.In addition,AN3 and AN4 were noncompetitive inhibitors of ACE,a-amylase and pancreatic lipase with Ki values of 0.33,0.37 mmol/L,0.39,0.41 mg/mL and 0.22,0.78 mg/mL.Conclusion:The extracts of Zijuan tea has the potential to anti-hypertensive,anti-hyperglycemic and anti-obesity.