文献类型：期刊文献

英文题名：Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone-nanohydroxyapatite composite

作者：Deng, Yi[1,2];Liu, Xiaochen[2];Xu, Anxiu[3];Wang, Lixin[4];Luo, Zuyuan[2];Zheng, Yunfei[1];Deng, Feng[3];Wei, Jie[5,6];Tang, Zhihui[1];Wei, Shicheng[1,2,3]

机构：[1]Peking Univ, Sch Hosp Stomatol, Dent Ctr 2, Lab Interdisciplinary Studies, Beijing 100081, Peoples R China;[2]Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100081, Peoples R China;[3]Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Beijing, Peoples R China;[4]Capital Med Univ, Beijing Shijitan Hosp, Dept Stomatol, Beijing, Peoples R China;[5]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[6]E China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2015

卷号：10

起止页码：1425

外文期刊名：INTERNATIONAL JOURNAL OF NANOMEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000349537000001)】；

基金：This work was supported by the Beijing Natural Science Foundation (7132124), State Key Development Program for Basic Research of China (grant 2007CB936103), and Peking University's 985 Grant.

语种：英文

外文关键词：polyetheretherketone; tenary; biocomposite; bioactivity; bone formation

摘要：As United States Food and Drug Administration-approved implantable material, carbon fiber-reinforced polyetheretherketone (CFRPEEK) possesses an adjustable elastic modulus similar to cortical bone and is a prime candidate to replace surgical metallic implants. The bioinertness and inferior osteogenic properties of CFRPEEK, however, limit its clinical application as orthopedic/dental implants. In this study, CFRPEEK-nanohydroxyapatite ternary composites (PEEK/n-HA/CF) with variable surface roughness have been successfully fabricated. The effect of surface roughness on their in vitro cellular responses of osteoblast-like MG-63 cells (attachment, proliferation, apoptosis, and differentiation) and in vivo osseointegration is evaluated. The results show that the hydrophilicity and the amount of Ca ions on the surface are significantly improved as the surface roughness of composite increases. In cell culture tests, the results reveal that the cell proliferation rate and the extent of osteogenic differentiation of cells are a function of the size of surface roughness. The composite with moderate surface roughness significantly increases cell attachment/proliferation and promotes the production of alkaline phosphatase (ALP) activity and calcium nodule formation compared with the other groups. More importantly, the PEEK/n-HA/CF implant with appropriate surface roughness exhibits remarkably enhanced bioactivity and osseointegration in vivo in the animal experiment. These findings will provide critical guidance for the design of CFRPEEK-based implants with optimal roughness to regulate cellular behaviors, and to enhance biocompability and osseointegration. Meanwhile, the PEEK/n-HA/CF ternary composite with optimal surface roughness might hold great potential as bioactive biomaterial for bone grafting and tissue engineering applications.