文献类型：期刊文献

英文题名：Engineering the Methylerythritol Phosphate Pathway and Using a Temporal Promoter for Enhanced Lycopene Production in Rhodobacter sphaeroides HY01

作者：He, Xinwei[1,2];Wang, Dan[1,2];Liu, Jing[1,2];Shi, Tong[1,2];Wang, Weishan[3];Chen, Biqin[4];Li, Dan[4];Zhang, Lixin[1,2,5];Tan, Gao-Yi[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China;[4]Inner Mongolia Kingdomway Pharmaceut Co Ltd, Hohhot 010206, Peoples R China;[5]Shanghai Collaborat Innovat Ctr Biomfg Technol SCI, Shanghai 200237, Peoples R China

年份：2024

卷号：72

期号：50

起止页码：28040

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20244917488435);WOS:【SCI-EXPANDED(收录号:WOS:001371308300001)】；

基金：This study was funded by the National Key Research and Development Program of China (2020YFA0907304 to G.- Y.T.); the National Natural Science Foundation of China (32370064 to G.-Y.T. and 32121005 to L.Z.); and 2023 Double World-class Project-Key Program-Intelligent Biomanufacturing to L.Z.

语种：英文

外文关键词：Rhodobactersphaeroides; coenzyme Q(10); terpenoids; lycopene; MEP pathway; temporal promoter

摘要：Rhodobacter sphaeroides HY01 is a high-yield strain for industrial production of coenzyme Q10 (Q10), indicating its potential for producing other terpenoids. However, the production of Q10 substantially depletes isoprene precursors, nearly eliminating other terpenoids like spheroidene and spheroidenone commonly found in wild-type R. sphaeroides. Lycopene was used as an example to demonstrate its potential for terpenoid biosynthesis. By refactoring the methylerythritol phosphate (MEP) pathway, such as overexpressing crtE and introducing crtI 4, lycopene production reached 126.1 mg/L in HY01. However, further overexpression of the deoxy-d-xylulose-5-phosphate synthase, 1-deoxy-d-xylulose 5-phosphate reductoisomerase, and isopentenyl-diphosphate isomerase genes led to strain degradation, significantly reducing lycopene production. Fine-tuning the engineered PrrAB two-component system, which upregulated the MEP pathway, increased lycopene production to 154.9 mg/L. Inspired by this result, a series of native promoters with varying strengths were identified and characterized through transcriptomic analysis during the late fermentation stage. Using these temporal promoters to control genes in the MEP pathway ultimately increased lycopene production to 283.1 mg/L, the highest reported in R. sphaeroides. These results underscore the potential of HY01 as a chassis for terpenoid biosynthesis.