文献类型：期刊文献

英文题名：Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles for enrichment of phosphopeptides prior to their determination by MALDI-TOF mass spectrometry

作者：Zhang, Lei[1];Gan, Yangyang[1];Sun, Haofan[1];Yu, Bohao[1];Jin, Xiaofeng[2];Zhang, Runsheng[3];Zhang, Weibing[1];Zhang, Lingyi[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]China Natl Qual Supervis & Testing Ctr Cu Pb Zn &, 1991 CuiHu Five Rd, Tongling 244000, Anhui, Peoples R China;[3]Shanghai Publ Secur Bur, Shanghai Res Inst Criminal Sci & Technol, Shanghai Key Lab Crime Scene Evidence, 803 ZhongShan North One Rd, Shanghai 200042, Peoples R China

年份：2017

卷号：184

期号：2

起止页码：547

外文期刊名：MICROCHIMICA ACTA

收录：;EI(收录号：20242616473200);WOS:【SCI-EXPANDED(收录号:WOS:000392632100021)】；

基金：This work was financially supported by the State Key Program of National Natural Science of China (21235005), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044), the National Natural Science Foundation of China (21475044), the model Project of the Research and Application of the Common Key Technology about Chemical Reagent of Basis Scientific Research (2015BAK44B00) and foundation of Shanghai Research Institute of Criminal Science and Technology.

语种：英文

外文关键词：Magnetic mesoporous carbon; Ferric oxide; Zirconia; Nanoparticles; Hydrophilic carbon material; Metal oxide affinity chromatography; Phosphopeptide; Enrichment; Human serum; MALDI-TOF MS

摘要：Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were synthesized from resol, ZrO(NO3)(2), ferric acetylacetonate, and triblock copolymer F127. The method involves a multi-component co-assembly strategy associated with direct carbonization. The resulting carbon material is shown to be useful as a metal oxide affinity chromatography (MOAC) material for enrichment of phosphopeptides owing to its large mesoporous (4.8 nm) surface area (442 m(2) g(-1)), large pore volume (0.37 cm(3) g(-1)) and excellent hydrophilicity. The metallic iron and ferric oxide particles modified on the mesoporous carbon exert a magnetic force and, in combination with the metallic zirconia, is a viable MOAC material for enrichment of low-abundance phosphopeptides. Because of metal chelation between metallic nanoparticles and the phosphate groups of phosphopeptides, the zirconia/magnetic mesoporous carbon displays high selectivity even at a phosphopeptide/nonphosphopeptide molar ratio of 1:500. As little as 1.5 fmol of phosphopeptides become detectable. The MOAC was successfully applied to the identification by MALDI-TOF MS of phosphopeptides in human serum and nonfat milk.