文献类型：期刊文献

英文题名：A polydopamine-based biomimetic multifunctional nanoplatform for multilayer imaging of cancer biomarkers carried by extracellular vesicles

作者：Zhou, Ze-Rui[1];Wang, Xiao-Yuan[1];Lv, Jian[1];Qian, Ruo-Can[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：145

期号：18

起止页码：6061

外文期刊名：ANALYST

收录：;EI(收录号：20204609485806);WOS:【SCI-EXPANDED(收录号:WOS:000568961600007)】；

基金：This research was supported by National Natural Science Foundation of China (21977031), National Science and Technology Major Project of China (2018ZX10302205), Shanghai Science and Technology Committee (19ZR1472300), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Biomarkers - Diseases - Gold nanoparticles - Diagnosis - Biomimetics

摘要：Cancer-derived extracellular vesicles (EVs) have attracted considerable attention for clinical diagnosis. However, a limiting factor in current EV assays is the ability to detect various EV cancer biomarkers expressed at different locations. Here, we report a biomimetic multifunctional nanoplatform for multilayer imaging of cancer biomarkers from the EV surface to the interior without complex pretreatment. Constructed from polydopamine-wrapped gold nanoparticles modified with multiple functional molecules, this nanoplatform can capture EVs from complex samples and target different EV cancer biomarkers for imaging analysis at the single-vesicle level. Combined with 96-well plates, this assay can distinguish cancer cell-derived EVs from normal ones in a high-throughput manner. Using serum samples, EVs from hepatocellular carcinoma (HCC) patients can be distinguished from healthy controls. This convenient workflow represents a promising tool for EV-based cancer diagnosis.