文献类型：期刊文献

英文题名：Analysis of the Regulatory Mechanism of Exogenous Iaa-Mediated Tryptophan Accumulation and Synthesis of Endogenous Iaa in Chlorococcum Humicola

作者：Chen, Xiurong[1,2]; Ye, Xiaoyun[1,2]; Yu, Xiao[1,2]; Zhao, Jiamin[1,2]; Song, Meijing[1,2]; Yin, Danning[1,2]; Yu, Jiayu[1,2]

机构：[1] National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230281955)

语种：英文

外文关键词：Acetic acid - Amino acids - Microorganisms - Molecular biology - pH - Polycyclic aromatic hydrocarbons - Wastewater treatment

摘要：The activated sludge method is widely used for the treatment of phenol-containing wastewater, which gives rise to the problem of toxic residual sludge accumulation. Indole-3-acetic acid (IAA) as a typical phytohormone can promote the removal of toxicity and the accumulation of biomass by microalgae. Proteomics analysis can reveal the mechanism of microalgae response to exogenous IAA. The results showed that the IAA concentration of 5×10-6 mol/L was most beneficial for Chlorococcum humicola to cope with the toxic stress in the sludge extract medium, enhance the antioxidant capacity and promote rubisco enzyme expression, and finally, it accumulated 1.57 times more protein than the control group. Exogenous IAA altered the relative abundance of various amino acids in Chlorococcum humicola cells, and proteomic analyses showed that exogenous IAA stimulated the algal cells to produce more IGP, indole, and serine, and tryptophan synthase (A0A383V983) was significantly up-regulated. IGP and serine were converted to tryptophan under the regulation of tryptophan synthase, and tryptophan served as a precursor for the synthesis of endogenous IAA, which also formed a promotional effect on the growth of Chlorococcum humicola. These findings have important implications for the treatment of toxic residual sludge while enriching for high-value amino acids. ? 2023, The Authors. All rights reserved.