文献类型：期刊文献

英文题名：Construction of cytokine reservoirs based on sulfated chitosan hydrogels for the capturing of VEGF in situ

作者：Wang, Chenmin[1,2];Yu, Yuanman[1,2];Chen, Han[1,2];Zhang, Shuang[1,2];Wang, Jing[1,2];Liu, Changsheng[2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2019

卷号：7

期号：11

起止页码：1882

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20191106638315);WOS:【SCI-EXPANDED(收录号:WOS:000461424000006)】；

基金：This work was supported by the financial support from the National Natural Science Foundation of China (No. 31330028, No. 31470923, and No. 31870953), the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), and the International Cooperation Project and Research Project of the Shanghai Science and Technique Committee (15520711100, 15DZ1942502). This study was also supported by the 111 Project (B14018). The authors also thank the Research Center of Analysis and Testing (ECUST) for their assistance in data collection and characterization.

语种：英文

外文关键词：Sulfur compounds - Cell engineering - Scaffolds (biology) - Binding energy - Endothelial cells - Blood vessels - Hydrogels - Mammals - Chitosan - Histology

摘要：Nutrients and oxygen are delivered mainly by blood vessels to nourish the cells and tissues in the body. Thus, biomaterials are processed by loading cytokines, such as vascular endothelial growth factors (VEGF), to facilitate angiogenesis in order to accelerate tissue regeneration. Nevertheless, the unpredictable biosecurity of exogenous cytokines is still a controversial issue for its clinical application. In this study, we constructed a kind of cytokine reservoir utilizing the binding affinity between heparin-like sulfate polysaccharide and endogenous growth factors. Two types of sulfated chitosan hydrogels, namely 6-O-sulfated chitosan (6-O-SCS) and 2-N, 6-O-sulfated chitosan (2-N, 6-O-SCS) hydrogels, were formed on the surface of the gelatin sponge matrix. The microstructure of the SCS-coated scaffolds is porous and interconnected, which is beneficial for cellular infiltration. Besides, human umbilical vein endothelial cells (HUVECs) can adhere and proliferate well on the surface of the scaffolds. Notably, sulfated chitosan-coated scaffolds exhibit an ability to capture VEGF in vitro & vivo, especially for the 2-N, 6-O-SCS-coated scaffold. It is also verified by mice models that sulfated chitosan-coated scaffolds result in a concentrated VEGF microenvironment in specific domains as cytokine reservoirs and induce mass microvessels after implantation into subcutaneous tissues. As such, the sulfated chitosan-coated scaffolds served as VEGF reservoirs to accelerate angiogenesis and wound healing. This beneficial strategy may be applicable to in situ tissue regeneration by capturing more cytokines and promoting healing.