文献类型：期刊文献

英文题名：Efficient Transformation of Linoleic Acid into 13(S)-Hydroxy-9,11-(Z,E)-octadecadienoic Acid Using Putative Lipoxygenases from Cyanobacteria

作者：Qi, Yi-Ke[1];Zheng, Yu-Cong[1];Zhang, Zhi-Jun[1];Xu, Jian-He[1]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：8

期号：14

起止页码：5558

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20201808595182);WOS:【SCI-EXPANDED(收录号:WOS:000526375000015)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 21536004, 21776085 & 21871085), the Natural Science Foundation of Shanghai (No. 18ZR1408400), National Key Research and Development Program of China (Nos. 2018YFC1706200 & 2019YFA09005000), and the Fundamental Research Funds for the Central Universities (No. 22221818014).

语种：英文

外文关键词：Linoleic acid; Biotransformation; 13S-Hydroxyoctadecadienoic acid; Lipoxygenase; Cyanobacteria

摘要：Enzymatic dioxygenation of unsaturated fatty acids by lipoxygenases (LOXs) represents a useful method in the transformation of bioresources for hydroperoxy and hydroxy fatty acids production. Difficulties in screening for optimal enzymes and in producing LOXs in sufficient quantity limit the large-scale application of LOXs. In this work, we identified three putative LOXs from Cyanobacteria, i.e., Ca-LOX from Calothrix sp. HK-06, Ri-LOX from Rivularia sp. PCC 7116, and Tb-LOX from Tolypothrix bouteillei VB521301. After heterologous expression in Escherichia coli and purification by HisTrap affinity chromatography, these LOXs showed high specific activities toward linoleic acid (73.1, 68.8, and 10.1 U mg(-1)protein, respectively). All three enzymes were identified as linoleate 13S-lipoxygenases. Ca-LOX and Ri-LOX are the highest-activity LOXs yet reported, and Ri-LOX gave a high titer of 38.3 U/mL on heterologous expression in E. coli. Consequently, by using Ri-LOX as an efficient biocatalyst with a subsequent reduction step, the renewable material linoleic acid was efficiently transformed into a value-added chemical, 13(S)-hydroxy-9,11-(Z,E)-octadecadienoic acid (13S-HODE), with an unprecedented space-time yield of 1008 g L-1 d(-1), indicating great potential of this LOX for sustainable production of 13-HODE and its derivatives.