文献类型：期刊文献

英文题名：Comparison of the cytotoxic impact of chlorfluazuron on selected insect and human cell lines

作者：Huang, Qingchun[1];Wu, Xiwei[1];Yu, Xiaoqin[1];Zhang, Lei[1];Lu, Miaoqing[1];Tao, Liming[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2015

卷号：34

期号：7

起止页码：1675

外文期刊名：ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY

收录：;EI(收录号：20152100868410);WOS:【SCI-EXPANDED(收录号:WOS:000357013700031)】；

基金：The present study was supported by the National Key Project for Basic Research of China (2010CB126101). We are grateful to L. Murdock from Purdue University USA, and D. Zhang from East China University of Science and Technology, China, for improvement of the English.

语种：英文

外文关键词：Chlorfluazuron; Cytotoxicity; Hek293; HepG2; Sf-21; Tn5B1-4

摘要：To gain new insight into the mechanism of selective cytotoxicity of benzoylureas as insecticides, the in vitro mode of action of chlorfluazuron was investigated on lepidopteran Tn5B1-4 and Sf-21 cells and human Hek293 and HepG2 cells. Chlorfluazuron inhibited the proliferation of Tn5B1-4 and Sf-21 cells with 50% inhibitory concentration values (IC50) of 4.96 mu M and 1.12 mu M at 48 h and 2.37 mu M and 1.76 mu M at 96 h, respectively, versus that of Hek293 and HepG2 cells with IC50 values >20 mu M. When transferred to chlorfluazuron-free medium, lepidopteran Tn5B1-4 and Sf-21 cells had a postinhibitory recovery development period within 24 h followed by a suppressed increase in cell viability, but human Hek293 and HepG2 cells showed an accelerated increase over their control level. Chlorfluazuron affected Tn5B1-4 and Sf-21 cells, with 1.8-fold decreases in the ratio of cellular N-acetylglucosamine (GlcNAc) level and protein content and 1.5-fold increases in the mitotic index and G(2)/M-phase arrest. Neither Hek293 nor HepG2 cells contained GlcNAc, and chlorfluazuron had no significant effects on the cell cycle and mitotic index of Hek293 and HepG2 cells. In conclusion, the differences between human and lepidopteran cell lines in the characteristic GlcNAc content, G(2)/M arrest in the cycle progress, and mitotic index of cells in response to chlorfluazuron may contribute to the selective toxicity of chlorfluazuron to lepidopteran cells. Environ Toxicol Chem 2015;34:1675-1682. (c) 2015 SETAC