文献类型：期刊文献

英文题名：High-Yield Biosynthesis of 3-Hydroxypropionic Acid from Acetate in Metabolically Engineered Escherichia coli

作者：Zhang, Wenrui[1];Zhang, Hongjun[1];Shang, Yanzhe[2];Luo, Yuanchan[1];Wu, Haizhen[1];Wu, Hui[1,2,3]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Dalian Univ Technol, Sch Bioengn, MOE Key Lab Biointelligent Mfg, Dalian 116024, Peoples R China;[3]China Natl Light Ind Council, Key Lab Biobased Mat Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：14

期号：5

起止页码：1654

外文期刊名：ACS SYNTHETIC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001473236200001)】；

基金：This study was supported by the Science and Technology Commission of Shanghai Municipality (24HC2820800) and partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Escherichia coli; acetate; 3-hydroxypropionicacid; syngas-derived; metabolic engineering; malonyl-CoA pathway

摘要：The third-generation biorefineries aimed at "carbon-negative" production of fuels and chemicals utilizing one-carbon molecules and renewable energy sources were raised to tackle the pressing climate change and food scarcity issues. Acetate derived from syngas fermentation, a viable nonfood carbon source, has recently been elevated in bulk chemicals biosynthesis. In this study, we successfully engineered Escherichia coli to produce 3-hydroxypropionic acid (3-HP) from acetate via the malonyl-CoA pathway. Initially, the constitutive promoter of the 3-HP biosynthetic pathway for efficient 3-HP production was screened in acetate-based medium. Then, efforts were focused on reducing the competition for malonyl-CoA by inhibiting the fatty acids (FAs) synthesis pathway. Furthermore, we enhanced the supply of NADPH and acetyl-CoA through cofactor engineering. The engineered strain ZWR18(M*DA) accumulated 5.53 g/L 3-HP, corresponding to a yield of 0.732 g/g, and achieved 97.60% of the theoretical yield. In whole-cell catalysis, ZWR18(M*DA) produced 23.89 g/L 3-HP with a yield of 0.734 g/g, reaching 97.87% of the theoretical yield. Utilizing syngas-derived acetate for whole-cell catalysis allowed ZWR18(M*DA) to accumulate 18.87 g/L 3-HP with a yield of 0.58 g/g. These results indicate that acetate from syngas can serve as a cost-effective and environmentally friendly alternative to traditional carbon sources, offering a sustainable biorefinery pathway for industrial biomanufacturing.