文献类型：期刊文献

英文题名：Improved production of the anticancer compound 1403C by glucose pulse feeding of marine Halorosellinia sp. (No. 1403) in submerged culture

作者：Kang, Li[1]; Cai, Menghao[1]; Yu, Chao[1]; Zhang, Yuanxing[1]; Zhou, Xiangshan[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2011

卷号：102

期号：22

起止页码：10750

外文期刊名：Bioresource Technology

收录：EI(收录号：20114314458545)

语种：英文

外文关键词：Batch cell culture - Fungi

摘要：Effects of different pulse fed-batch methods on production of the anti-cancer compound 1403C by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403) in a 5-L bioreactor were investigated. Since high glucose concentrations improved mycelial growth but inhibited 1403C production, the cultures were pulse fed with glucose solutions to keep the residual glucose lower than 4g/L but higher than 0.5g/L during rapid growth phase (0-50h). In this way, a maximum dry biomass, 1403C production and yield coefficient (Y1403C/X) of up to 4.5g/L, 2.64g/L and 0.59g/g dry cell weight, respectively were achieved. These values are 22.7%, 98.0% and 61.4%, respectively higher than those obtained with batch cultures. This strategy is valuable for fermentation scale-up of Halorosellinia sp. (No. 1403) for 1403C production, and might also be applicable to other marine fungi cultures. ? 2011 Elsevier Ltd.