文献类型：期刊文献

英文题名：High-throughput screening of high lactic acid-producing Bacillus coagulans by droplet microfluidic based flow cytometry with fluorescence activated cell sorting

作者：Zhu, Xu-Dong[1];Shi, Xiang[1];Wang, Shu-Wen[2];Chu, Ju[1];Zhu, Wei-Hong[2];Ye, Bang-Ce[1];Zuo, Peng[1];Wang, Yong-Hong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2019

卷号：9

期号：8

起止页码：4507

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20190906549338);WOS:【SCI-EXPANDED(收录号:WOS:000459896700046)】；

基金：This study was financially supported by the National Science Foundation for Young Scientists of China (31700038), the National Natural Science Foundation of China (21878084) and the National Key Research and Development Program (2017YFB0309302).

语种：英文

外文关键词：Fermentation - Bacteriology - Microfluidics - Drops - Throughput - Fluorescence

摘要：A high-throughput screening system based on droplet microfluidic sorting was developed and employed for screening of high lactic acid-producing Bacillus coagulans. In this system, water-in-oil-in-water (W/O/W) droplets, which were similar to 12 pL in volume were used as picoliter-reactors for lactic acid fermentation. A fluorescent sensor was developed and used for monitoring pH which indicated the production of lactic acid. After fermentation, fluorescence activated cell sorting was performed with high sensitivity and speed. Using this microfluidic high-throughput screening system, we found a mutant with a yield of 76 g L-1 lactic acid which was 52% higher than its parent strain with a screening throughput exceeding 10(6) clones per h.