文献类型：期刊文献

中文题名：白花蛇舌草总多酚纯化工艺及体外抗炎活性研究

英文题名：Purification Process and in Vitro Anti-inflammatory Activity of Total Polyphenols from Hedyotis Diffusa Willd

作者：朱义越[1,2,3];陆晔晗[1,2,3];常芮宁[1,2,3];宋艺[1,2,3];王艾雯[1,2,3];王春丽[1,2,3];郭美珠[4];张春芳[5]

机构：[1]华东理工大学药学院,上海200237;[2]制药工程与过程化学教育部工程研究中心,上海200237;[3]上海市新药设计重点实验室,上海200237;[4]上海市宝山区中西医结合医院,上海201900;[5]厦门大学附属翔安医院中医科,福建厦门361005

年份：2025

卷号：42

期号：7

起止页码：1091

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：;北大核心:【北大核心2023】；

基金：上海市宝山区科技委员会科技创新专项(20-E-15);大学生创新创业训练计划(X202210251134)。

语种：中文

中文关键词：白花蛇舌草;大孔树脂;纯化;多酚;体外抗炎

外文关键词：Hedyotis diffusa Willd;macroporous resin;purification;polyphenols;in vitro anti-inflammatory

摘要：目的探讨大孔树脂对于白花蛇舌草总多酚的最佳纯化工艺条件及其体外抗炎效果。方法采用超声波辅助提取法提取白花蛇舌草总多酚,静态吸附-解吸平衡动力学实验研究不同大孔树脂吸附效果,筛选最佳纯化工艺条件,并通过5-脂氧合酶(5-lipoxygenase,5-LOX)和环氧合酶-2(cyclooxygenase-2,COX-2)抑制实验评价其抗炎活性。结果采用HPD-826型大孔树脂纯化白花蛇舌草总多酚在上样液浓度为1.5 mg·mL^(-1)、pH=5.0、上样体积90 mL、上样流速为1.0 mL·min^(-1)、洗脱液乙醇体积分数为70%、洗脱剂体积80 mL、洗脱流速为1.0 mL·min^(-1)条件下纯化效果最好,白花蛇舌草总多酚纯度由(27.13±1.36)%提升到(63.08±0.83)%,提高了1.32倍。总多酚浓度为20 mg·mL^(-1)的白花蛇舌草醇提液,对5-LOX活性抑制率可达到(57.15±7.16)%;总多酚浓度为3.2 mg·mL^(-1)的白花蛇舌草醇提液对COX-2活性抑制率可达到(83.49±2.48)%。结论大孔树脂纯化技术可以提高白花蛇舌草总多酚的纯度,其对炎症介导的5-LOX和COX-2均具有抑制作用,且对COX-2的抑制效果更佳。
OBJECTIVE To explore the optimal purification process condition of macroporous resin on the total polyphenolic compounds of Hedyotis diffusa,and to investigate its in vitro anti-inflammatory effect.METHODS The total polyphenols in Hedyotis diffusa were extracted by ultrasound-assisted extraction,and the adsorption effect of different macroporous resins was investigated in static adsorption-desorption equilibrium kinetic experiments to screen the optimal purification process conditions,and the anti-inflammatory activity was evaluated by the inhibition of 5-lipoxygenase(5-LOX)and cyclooxygenase-2(COX-2)assays.RESULTS The purification of total polyphenols from Hedyotis diffusa using HPD-826 macroporous resin was found to be most effective under the following conditions:sample concentration of 1.5 mg·mL^(-1),pH of 5.0,sample volume of 90 mL,sample flow rate of 1.0 mL·min^(-1),elution solution ethanol volume fraction of 70%,elution solution volume of 80 mL,and elution flow rate of 1.0 mL·min^(-1).Under these conditions,the purity of total polyphenols from Hedyotis diffusa increased from(27.13±1.36)%to(63.08±0.83)%,representing a 1.32-fold improvement.The ethanolic extract of Hedyotis diffusa with a total polyphenol concentration of 20 mg·mL^(-1) exhibited a(57.15±7.16)%inhibition rate against 5-LOX activity,while the ethanolic extract with a total polyphenol concentration of 3.2 mg·mL^(-1) demonstrated an(83.49±2.48)%inhibition rate against COX-2 activity.CONCLUSION The macroporous resin purification technology can enhance the purity of total polyphenols from Hedyotis diffusa.It exhibits inhibitory effects on inflammation-mediated 5-LOX and COX-2,with a more pronounced inhibitory effect on COX-2.