文献类型：期刊文献

中文题名：灭多威对果蝇胚胎S2和人肝癌HepG2细胞的遗传毒性研究

英文题名：Evaluation on genotoxicity of methomyl against Drosophila Schneider 2 and Human hepatocellular carcinoma G2 cells

作者：李荻秋[1];吴鸿杰[1];项光刚[1];徐白雪[1];陶黎明[1]

机构：[1]华东理工大学药学院 上海市化学生物学重点实验室,上海200237

年份：2014

卷号：16

期号：5

起止页码：517

中文期刊名：农药学学报

外文期刊名：Chinese Journal of Pesticide Science

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金："十二五"国家科技支撑计划项目(2011BAE06B04-1)

语种：中文

中文关键词：灭多威;果蝇胚胎S2细胞;人肝癌HepG2细胞;细胞活力;DNA损伤

外文关键词：methomyl;S2 cells;HepG2 cells;cell viability;DNA damage

摘要：灭多威对环境中非靶标生物的毒性作用广受关注。选用果蝇胚胎S2细胞和人肝癌HepG2细胞为研究对象,采用噻唑蓝(MTT)细胞活力测定、单细胞彗星电泳和磷酸化组蛋白(γH2AX)免疫荧光印迹等方法研究了灭多威的细胞毒性。结果表明:随着灭多威浓度的升高,S2和HepG2细胞活力均呈逐渐下降趋势;两种细胞均出现显著的彗星现象,且彗星尾长增加,尾部面积增大;γH2AX阳性细胞百分率逐渐增大。表明果蝇S2和人HepG2细胞DNA受损程度随着灭多威剂量的增大而加重,灭多威可诱导细胞DNA双链断裂,最终导致细胞凋亡。灭多威具有潜在的基因毒性,长时间接触会损害人类健康。
As a widely used carbamate insecticide, the toxic effects of methomyl on the environmental non-target organisms have been paid more attention. The genotoxicity of methomyl against Drosophila Schneider 2 （ S2 ） cells and Human hepatocellular carcinoma G2 （ HepG2 ） cells was investigated by using MTT （ 3-4, 5-dimethyl-2-thiazolyl ）-2, 5-diphenyl-2-H-tetrazolium bromide ） assay procedure, single-cell comet electrophoresis and γH2AX （ phosphorylated histones H2AX） immunofluorescence. The results showed that as the concentrations of methomyl were increased, cell viability of S2 and HepG2 were gradually declined;cell comet phenomena were induced significantly, the length of comet tail became longer and the tail area increased; the percentage of γH2 AX positive cells were increased gradually. The results suggested that the extent of DNA damage in S2 and HepG2 cells was concentration-dependent on the doses of methomyl, and methomyl could induce DNA double-strand breaks and cause cell apoptosis. Methomyl possessed potentially genotoxicity on insect and human, the＆nbsp;prolonged exposure to methomyl might be harmful for human health.