文献类型：期刊文献

英文题名：The antimicrobial activity of Cbf-K16 against MRSA was enhanced by β-lactamantibiotics through cell wall non-integrity

作者：Li, Bo[1];Kang, Wei[1];Liu, Hanhan[1];Wang, Yanrong[1];Yu, Changzhong[1];Zhu, Xinyi[1];Dou, Jie[1];Cai, Haibo[2];Zhou, Changlin[1]

机构：[1]China Pharmaceut Univ, Sch Life Sci & Technol, State Key Lab Nat Med, 24 Tong Jia Xiang, Nanjing 210009, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：39

期号：7

起止页码：978

外文期刊名：ARCHIVES OF PHARMACAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000379692900012)】；

基金：This research was financially supported by the Priority Academic Development Program of Jiangsu Higher Education Institutions (PAPD), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, and College Students Innovation Project for the R&D of Novel Drugs.

语种：英文

外文关键词：Cbf-K-16; Antimicrobial activity; Ceftazidime; Ampicillin; MRSA; Synergistic effect

摘要：Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as one of the most important pathogens both in health care and in community-onset infections. Cbf-K-16, a cathelicidin-like antimicrobial peptide, presented broad antimicrobial activity during our previous studies. We evaluated the potential for synergy of Cbf-K-16 with ceftazidime/ampicilin against MRSA, which was resistant to these two antibiotics with the minimum inhibitory concentration more than 64 mu g/ml. The combinations showed a synergistic effect by a checkerboard assay with a fractional inhibitory concentration index a parts per thousand currency sign0.5. The killing curves of the combination treatment against MRSA showed that CFU counts decreased rapidly within 4 h by almost five logs, while single medication groups and the control group exhibited little inhibitory effect. In addition, in a mice bacteremia model, studies indicated that the combination treatment significantly prolonged the survival time of mice infected with MRSA, with a death protection rate of 80 %. Flow cytometry analysis and transmission electron microscopy indicated that combination-treated MRSA was completely ruptured with the cellular contents leaked out. The synergistic effect showed that Cbf-K-16 selectively killed cells with non-integrity induced by cell wall inhibition antibiotics, suggesting that Cbf-K-16 is a potential therapeutic agent and adjuvant for antimicrobial chemotherapy.