文献类型：期刊文献

英文题名：Hybrid monolithic columns with nanoparticles incorporated for capillary electrochromatography

作者：Lei, Wen[2];Zhang, Ling-Yi[2];Wan, Li[2];Shi, Bian-Fang[1];Wang, Yan-Qin[1];Zhang, Wei-Bing[2]

机构：[1]E China Univ Sci & Technol, Res Inst Ind Catalysis, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2012

卷号：1239

起止页码：64

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20121814986224);WOS:【SCI-EXPANDED(收录号:WOS:000303965100007)】；

基金：We gratefully acknowledge the support of the National Natural Science Foundation for Young Scientists of China (No. 21105027), the Fundamental Research Funds for the Central Universities of China (WK1014042), and the Science and Technology Commission of Shanghai Municipality (10dz2220500).

语种：英文

外文关键词：Nanoparticles; Hybrid monoliths; CEC; Mixed-mode stationary phases

摘要：The core-shell silica nanoparticles Fe3O4@SiO2/NH2, wormlike and hexagonal SBA-15 silica were incorporated into polymethacrylate monolithic columns containing butyl methacrylate (BMA) and ethylene dimethacrylate (EDMA), respectively to develop novel stationary phases with mixing mechanism of reverse phase and ion exchange. Experimental conditions including types of nanoparticles, dispersion pattern, nanoparticles concentration, column placement mode, and reaction temperature were optimized for simple and stable column preparation. The poly(BMA-EDMA-Fe3O4@SiO2/NH2) and poly( BMA-EDMA-SBA-15/NH2) (both wormlike and hexagonal shape nanoparticles) monolithic columns were evaluated with mixture of organic acids as sample in capillary electrochromatography (CEC) mode and the relative column efficiency reaches 290,000 plates/m. The results indicate that the incorporation of nanoparticles with various shapes enhances both selectivity and column efficiency due to high specific surface area of nanoparticles and mixing separation mechanism. In addition, poly( BMA-EDMA-Fe3O4@SiO2/NH2) monolith capillary column was applied to separation of aqueous extract of rhizoma gastrodiae and showed great potential in the method development of complex samples. (C) 2012 Elsevier B.V. All rights reserved.