文献类型：期刊文献

英文题名：Facile synthesis of zwitterionic polymer-coated core-shell magnetic nanoparticles for highly specific capture of N-linked glycopeptides

作者：Chen, Yajing[1];Xiong, Zhichao[1];Zhang, Lingyi[1];Zhao, Jiaying[1];Zhang, Quanqing[2];Peng, Li[1];Zhang, Weibing[1,2];Ye, Mingliang[2];Zou, Hanfa[2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R& Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

年份：2015

卷号：7

期号：7

起止页码：3100

外文期刊名：NANOSCALE

收录：;EI(收录号：20150800537860);WOS:【SCI-EXPANDED(收录号:WOS:000349473200034)】；

基金：Financial support is gratefully acknowledged from the National Natural Science Foundation of China (21235006), the National Natural Science Foundation of China (no. 21475044), the Science and Technology Commission of Shanghai Municipality (12JG0500200), the Creative Research Group Project by NSFC (2132106), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044), the China State Key Basic Research Program Grant (2013CB911202, 2012CB-910601 and 2012CB-910101), the Analytical Method Innovation Program of MOST (2012M030900), and the Knowledge Innovation Program of DICP to H.F.Z.

语种：英文

外文关键词：Peptides - Precipitation (chemical) - Liquid chromatography - Silica nanoparticles - Ammonium hydroxide - Nanomagnetics - Magnetic separation - Synthesis (chemical) - Hydrophilicity

摘要：Highly selective and efficient capture of glycosylated proteins and peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. Recently, hydrophilic interaction liquid chromatography (HILIC)-based functional materials have been extensively utilized for glycopeptide enrichment. However, the low amount of immobilized hydrophilic groups on the affinity material has limited its specificity, detection sensitivity and binding capacity in the capture of glycopeptides. Herein, a novel affinity material was synthesized to improve the binding capacity and detection sensitivity for glycopeptides by coating a poly(2-(methacryloyloxy) ethyl)dimethyl-(3-sulfopropyl) ammonium hydroxide (PMSA) shell onto Fe3O4@SiO2 nanoparticles, taking advantage of reflux-precipitation polymerization for the first time (denoted as Fe3O4@SiO2@PMSA). The thick polymer shell endows the nanoparticles with excellent hydrophilic property and several functional groups on the polymer chains. The resulting Fe3O4@SiO2@PMSA demonstrated an outstanding ability for glycopeptide enrichment with high selectivity, extremely high detection sensitivity (0.1 fmol), large binding capacity (100 mg g(-1)), high enrichment recovery (above 73.6%) and rapid magnetic separation. Furthermore, in the analysis of real complicated biological samples, 905 unique N-glycosylation sites from 458 N-glycosylated proteins were reliably identified in three replicate analyses of a 65 mu g protein sample extracted from mouse liver, showing the great potential of Fe3O4@SiO2@PMSA in the detection and identification of low-abundance N-linked glycopeptides in biological samples.