文献类型：期刊文献

英文题名：Control of fungal morphology for improved production of a novel antimicrobial alkaloid by marine-derived fungus Curvularia sp IFB-Z10 under submerged fermentation

作者：Yang, Jun[1,2];Jiao, Rui-Hua[3];Yao, Ling-Yun[1,2];Han, Wen-Bo[3];Lu, Yan-Hua[1,2];Tan, Ren-Xiang[3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Box 283,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Nanjing Univ, Inst Funct Biomol, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China

年份：2016

卷号：51

期号：2

起止页码：185

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20155001658208);WOS:【SCI-EXPANDED(收录号:WOS:000370100200004)】；

基金：This work is financially supported by the National High Technology Research and Development Program of China (2013AA092901), the Fundamental Research Funds for the Central Universities, as well as the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204).

语种：英文

外文关键词：Curvularia sp IFB-Z10; Curvulamine; Morphology; Marine fungus; Submerged fermentation

摘要：Curvulamine is a novel alkaloid with strong antimicrobial activity produced by the marine-derived fungus Curvularia sp. IFB-Z10. In this work, the effects of different carbon and nitrogen source on curvulamine production were firstly investigated. Different morphologies were formed and pellet-like growth of fungi was found positive for the curvulamine formation, and sucrose, yeast extract and sodium nitrate revealed most suitable for curvulamine formation. Subsequently, four seed culture methods were employed to examine the linkage between pellet shape and curvulamine production. The mean pellet diameter was significantly decreased and the production was improved to 17.97 mg/L by method M3, which used baffled shake flask and glass homogenizer to prepare the homogenized seed broth. Furthermore, microparticle-based morphology engineering was used to control the pellet shape and enhanced the curvulamine production. By supplementation of talc powder (200 mesh, 5 g/L), the mean pellet diameter dramatically decreased and the fermentation titer reached 33.05 mg/L, which was about 1.9-fold of the control (17.21 mg/L). In addition, the fermentation process was scaled up in a 5-L bioreactor and the curvulamine production reached 21.63 mg/L, which was about 3.3-fold of the control (6.58 mg/L). The results obtained provided a new approach to enhance curvulamine production. (C) 2015 Published by Elsevier Ltd.