文献类型：期刊文献

英文题名：Membrane-bound pyrroloquinoline quinone-dependent dehydrogenase in Gluconobacter oxydans M5, responsible for production of 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose

作者：Yang, Xue-Peng[1,2]; Wei, Liu-Jing[1]; Lin, Jin-Ping[1]; Yin, Bo[1]; Wei, Dong-Zhi[1,3]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China; [2] College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China; [3] East China University of Science and Biotechnology, Mei-Long 130, Shanghai 200237, China

年份：2008

卷号：74

期号：16

起止页码：5250

外文期刊名：Applied and Environmental Microbiology

收录：EI(收录号：20083511493034)

语种：英文

外文关键词：Alcohols - Microbiology

摘要：A membrane-bound protein purified from Gluconobacter oxydons M5 was confirmed to be a pyrroloquinoline quinone-dependent D-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-D-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol. Copyright ? 2008, American Society for Microbiology. All Rights Reserved.