文献类型：期刊文献

中文题名：Influence of initial glucose concentration on seed culture of sodium gluconate production by Aspergillus niger

作者：Xiu Liu[1];Xiwei Tian[1];Haifeng Hang[1];Wei Zhao[2];Yonghong Wang[1];Ju Chu[1]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,130 Meilong Road,Shanghai,200237,People’s Republic of China;[2]Shan Dong Fuyang Biological Technology Co.,Ltd,Shanghai,China

年份：2017

卷号：4

期号：1

起止页码：8

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：supported by the National Basic Research Program of China(973 Project)(No.2013CB733600);the National High Technology Research and Development Program 863(No.2015AA021005).

语种：英文

中文关键词：Aspergillus niger;Sodium gluconate;Initial glucose concentration;Osmotic pressure;Seed culture;Metabolite profiling

摘要：Background In general,high-quality seed is the prerequisite of an efficient bioprocess.However,in terms of sodium gluconate production by Aspergillus niger,reports have seldom focused on seed culture with rational optimization by process analysis technology,especially for carbon source effects.In this study,based on the online physiological parameter of oxygen uptake rate(OUR),and intracellular metabolite profiling,as well as cell morphology analysis,the effects of different initial glucose concentrations on seed culture by A.niger were investigated.Results The optimum initial glucose concentration was 300 g/L,corresponding to 1900 mOsm/kg,with OUR level about 70%higher than other conditions.Besides,the cells from optimized seed culture accumulated more osmoprotectants of alanine and glutamate.Interestingly,high glucose concentration could induce glucose oxidase(GOD)activity possibly by affecting the synthesis of histidine,one key component of active site of GOD.Prominently,the fermentation yield using the optimized seed culture was up to 1.198 g/g,99%of the theoretical value,which was the best in literature.Conclusion The initial glucose concentration appropriately 300 g/L in seed cultivation was determined to be the most optimal.Further,this study would be helpful for guiding sodium gluconate production on industrial scale.