文献类型：期刊文献

英文题名：Oxygen uptake rate (OUR) guided nitrogen control strategy for improving nemadectin production by Streptomyces cyaneogriseus ssp. noncyanogenus

作者：Song, Xiaoqing[1];Zhang, Zishu[1];Yu, Junxiong[1];Zhang, Yun[1];Xue, Jiayun[1];Guo, Yuanxin[1];Liang, Jianguang[2];Ren, Min[3];Ling, Qingyun[3];Mohsin, Ali[1];Qian, Jiangchao[1];Wang, Zejian[1];Wang, Yonghong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Changshu Inst Technol, Sch Biol & Food Engn, Changshu 215500, Peoples R China;[3]Jiangsu Weiling Pharmaceut Co Ltd, Huaian, Jiangsu, Peoples R China

年份：2025

卷号：150

起止页码：248

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20250417756539);WOS:【SCI-EXPANDED(收录号:WOS:001409689900001)】；

基金：This work was financially supported by grants from National Key R&D Program of China (No. 2021YFC2101000) , National Natural Science Foundation of China [No. 32071471] , Shanghai Scientific and Technological Innovation Action Plans-Scientific Instrument Development, China (grant No. 21142201300, and No. 22142201000) , the Fundamental Research Funds for the Central Universities (No. 222201714024) , and Innovation and Intelligence 111 Plan.

语种：英文

外文关键词：Nemadectin; S. cyaneogriseus; On-line parameters; Real-time monitoring; Fed-batch fermentation

摘要：Oxygen supply and oxygen uptake rate (OUR) significantly influences both the physiological state of cells and nemadectin biosynthesis by Streptomyces cyaneogriseus ssp. noncyanogenus. In this study, a real-time monitoring approach for nemadectin fermentation was developed for the first time by an online OUR control strategy. The effect of OUR levels during the mycelium differentiation phase on nemadectin biosynthesis was studied and optimized by adjusting feeding rates of (NH4)2SO4. Results showed that controlling the OUR at approximately 17.5 mmol center dot L- 1 center dot h- 1 during the mycelium differentiation phase effectively enhanced nemadectin production, reaching 2805.1 mu g center dot mL-1, making a 129.5 % increase compared to the control. This OUR control strategy was successfully scaled-up to a 500 L pilot scale, achieving the highest ever nemadectin production of 2867.3 mu g center dot mL- 1. These findings demonstrate that this OUR control strategy provides an effective and scalable approach for industrial nemadectin production.