文献类型：期刊文献

英文题名：Medium screening and optimization for photoautotrophic culture of Chlorella pyrenoidosa with high lipid productivity indoors and outdoors

作者：Wang, Weiliang[1];Han, Feifei[1];Li, Yuanguang[1];Wu, Yinsong[1];Wang, Jun[2];Pan, Ronghua[2];Shen, Guomin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiaxing Zeyuan Bioprod Ltd Co, Jiaxing 314006, Peoples R China

年份：2014

卷号：170

起止页码：395

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20144600181703);WOS:【SCI-EXPANDED(收录号:WOS:000341357600050)】；

基金：We thank Dr. Jianhua Fan, Dr. Minxi Wan and Mr. Zhen Zhang for revising the manuscript. This research was funded by the National Basic Research Program of China (973Program: 2011CB200904 and 2011CB200903), the National High Technology Research and Development Program of China (863 Program: 2012AA050101, 2013AA065804), National Natural Science Foundation of China (31372548) and the National Key Technologies R&D Program (2011BAD14B02 and 2011BAD23B04).

语种：英文

外文关键词：Chlorella pyrenoidosa; Heterotrophic seed; Medium screening and optimization; Indoors and outdoors; Cultivation

摘要：Medium screening and optimization is one of the most important preconditions for photoautotrophic cultivation of microalgae. Although, it has been widely conducted indoors, little work performed outdoors. There are enormous differences between indoor and outdoor conditions, especially for light intensity, temperature and their diurnal or annual fluctuations, which would greatly influence microalgae growth. No data shows whether the differences would lead to different results on medium screening and optimization. In present study, medium screening for the photoautotrophic cultivation of Chlorella pyrenoidosa was carried out indoors and outdoors firstly, and then the selected medium was optimized. The results showed that F-Si medium is the optimum both under indoor and outdoor conditions. Based on F-Si medium, nutrients were optimized as follows: NaNO3 500 mg l (1), NaH2PO4 center dot 2H(2)O 7.7 mg l (1) and FeCl3 center dot 6H(2)O 6.30 mg l (1). With the optimized medium, the biomass, lipid content and productivity were all significantly higher both indoors and outdoors. (C) 2014 Elsevier Ltd. All rights reserved.