文献类型：期刊文献

英文题名：Controllable immobilization of proteins in quenched spherical polyelectrolyte brushes as observed by fluorescence spectroscopy and small angle X-ray scattering

作者：Ye, Zhishuang[1];Li, Li[1];Zhao, Fang[1];Han, Haoya[1];Tian, Yuchuan[1];Wang, Yunwei[1];Yang, Qingsong[1];Fan, Wenting[1];Guo, Xuhong[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Engn Res Ctr Mat Chem Engn Xinjiang Bingtuan, Xinjiang 832000, Peoples R China

年份：2018

卷号：56

期号：23

起止页码：1577

外文期刊名：JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS

收录：;EI(收录号：20184105912668);WOS:【SCI-EXPANDED(收录号:WOS:000449662800004)】；

基金：We sincerely thank Professor Matthias Ballauff and Professor Lu Yan for their valuable guidance and discussion. Financial support by NSFC Grants (21476143, 51773061, and 5171101370), 111 Project Grant (B08021), and the technical support by Shanghai Synchrotron Radiation Facility are gratefully acknowledged.

语种：英文

外文关键词：controllable immobilization of protein; fluorescence spectroscopy; small angle X-ray scattering; spherical polyelectrolyte brush

摘要：The immobilization of lysozymes (pI = 11) onto anionic spherical polyelectrolyte brushes (SPB) which consist of a solid polystyrene core and a densely grafted poly(styrene sulfonate) (PSS) shell was systematically studied by fluorescence spectroscopy and small angle X-ray scattering. Results show that the capture of lysozyme by PSS brush is a dynamic process, which involves a quick agglomeration stage and a slow rearrangement one. And lysozyme inclines to immobilize in the inner layer of the brush, and saturation of lysozyme adsorption onto the SPB is gradually reached as the protein concentration increases, proceeding from the inside to the outside of the brush layers. As increasing the pH and ionic strength, the lysozyme previously adsorbed will be partially released and migrate from the inner to the outer layer of SPB. Last competitive adsorption tests between lysozyme and BSA or beta-glucosidase were performed, indicating that besides electrostatic interaction counterion release force also plays an important role in protein adsorption. SPB was proved to be ideal candidate for controllable immobilization of protein, which can be extended into various applications, such as drug delivery and protein separation. (c) 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2018, 56, 1577-1588