文献类型：期刊文献

英文题名：Harnessing Paecilomyces variotii to remove lignocellulose-derived inhibitors: potential evaluation, lignocellulosic biorefining route design, and transcriptome analysis

作者：Zhang, Bin[1];Wu, Shinan[1];Chen, Agustian[1];Yi, Xia[2];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Changzhou Univ, Natl Local Joint Engn Res Ctr Biomass Refining & H, Changzhou 213164, Peoples R China

年份：2025

卷号：5

期号：4

起止页码：1579

外文期刊名：SYSTEMS MICROBIOLOGY AND BIOMANUFACTURING

收录：EI(收录号：20253519055495);WOS:【ESCI(收录号:WOS:001556002300001)】；

基金：This work was supported by the National Key R&D Program of China (2023YFA0914400), the National Natural Science Foundation of China (32301269), the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900), and the Key Technology R&D Program of the Science and Technology Com-mission of Shanghai Municipality (25HC2820200).

语种：英文

外文关键词：Biodetoxification; Paecilomyces variotii; Lignocellulose; Lactic acid; Transcriptome analysis

摘要：Discovery and identification of robust biodetoxification strain is crucial for the sustainable and efficient operation of lignocellulosic biorefining process. Paecilomyces variotii FN89, a recently isolated mesophilic filamentous fungi, was herein shown to be able to biodegrade lignocellulose-derived inhibitors including furfural (1.5 g/L), 5-hydroxymethylfurfural (4 g/L), acetic acid (4 g/L), hydroxybenzaldehyde (0.2 g/L), syringaldehyde (0.2 g/L), and vanillin (1.5 g/L) efficiently and completely. P. variotii FN89 was adapted to mixed inhibitors and relatively low dissolved oxygen conditions, which can detoxify both the highly viscosity hydrolysate and solid biomass with the well preserve of fermentable sugars and no addition of any nutrients. Two biorefinery chains involving biodetoxification process were thus established to cope with different forms of pretreated biomass for cellulosic lactic acid production. The cellulosic lactic acid titers were above 100 g/L from 25% (w/w) solids loading pretreated wheat straw. The global transcriptome analysis of P. variotii FN89 in the presence of mixed inhibitors suggested that the glycolysis pathway and pentose phosphate pathway were repressed while tricarboxylic acid cycle was enhanced, ensuring the complete degradation of the inhibitors-derived intermediates and efficient energy supply. This study provided a unique and practical biodetoxification strain for lignocellulosic biorefinery, as well as enriched the knowledge of the molecular basis of lignocellulose-derived inhibitors tolerance and carbohydrates metabolism of P. variotii.