文献类型：期刊文献

中文题名：喹啉腈荧光微球构建及在荧光免疫层析中对SAA的检测应用

英文题名：Construction of Quinoline-Malononitrile Fluorescent Microspheres for the Detection of SAA via Fluorescent Immunochromatography

作者：李强[1,2];徐建新[1];郭志前[2]

机构：[1]上海奥普生物医药股份有限公司,上海即时检测医学工程技术研究中心,上海200201;[2]华东理工大学化学与分子工程学院,精细化工研究所,上海200237

年份：2022

卷号：48

期号：4

起止页码：419

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：国家自然科学基金面上项目(21878087)。

语种：中文

中文关键词：聚集诱导发光;喹啉腈;荧光微球;荧光免疫层析;血清淀粉样蛋白A

外文关键词：aggregation-induced emission;quinoline-malononitrile;fluorescent microspheres;fluorescent immunochromatography;serum amyloid A

摘要：基于聚集诱导发光(AIE)喹啉腈(QM)染料的衍生物QM-OH制备了乳胶荧光微球QM-OH@PS-COOH(羧基聚苯乙烯),通过扫描电子显微镜等表征仪器研究了微球的形貌结构,并进一步将该荧光微球应用于荧光免疫层析法检测中,实现了对临床样本中血清淀粉样蛋白A(SAA)含量变化的灵敏检测。研究结果表明:QM-OH@PS-COOH荧光微球大小均一、形状规整,与市售荧光微球对比,其密度、固含量、羧基含量基本一致;该荧光微球体系不仅可以定性区分炎症感染,而且能对SAA含量进行定量分析,实现了对炎症感染的有效评估和监控。因此,基于QM-OH@PS-COOH的检测平台可用于早期疾病检测的筛查,特别是用于即时检测领域中的自我检测。
Serum amyloid A(SAA)is an acute-phase protein mainly produced by the liver in response to proinflammatory cytokines.SAA genes and proteins are significantly activated during the acute phase response,which comprises a number of phenomena that occur in the presence of inflammation and infection,increased temperature and hormonal and metabolic alterations.Therefore,SAA is a sensitive indicator of inflammation in the early stage of infectious diseases,which is important for diagnosis,evaluation,monitoring and treatment of inflammation.Fluorescent immunochromatography is one of the most popular strategies for point-of-care testing(POCT),which is capable of rapid screening for disease detection.Fluorescent microspheres QM-OH@PS-COOH were obtained from aggregationinduced emission(AIE)quinoline-malononitrile(QM)derivatives QM-OH.The morphology and structure of these QM fluorescent microspheres were characterized by scanning electron microscopy et al.Finally,these fluorescent microspheres were utilized to detect for SAA concertation in clinical samples via fluorescent immunochromatography.The results showed that QM-OH@PS-COOH had uniform sizes with regular shapes.Compared with commercial fluorescent microspheres,these AIE microspheres had similar density,solid content and carboxyl content.The QMOH@PS-COOH system exhibited the detection of SAA with high sensitivity in clinical samples via fluorescent immunochromatography.Thus,this new type of QM fluorescent microspheres could be employed as an important tool for clinical diagnosis,enabling quantitatively analyze and monitor the concentration of SAA during the inflammation process.Therefore,we believe that these detection platforms based on QM-OH@PS-COOH can serve as a screening platform for early disease detection,especially self-testing in POCT.