文献类型：期刊文献

英文题名：Sequence-activated chemiluminescence probes: programmable illumination for high-contrast in vivo sensing and imaging

作者：Zhang, Yutao[1];Li, Juan[1];Zhu, Wei-Hong[1];Guo, Zhiqian[1,2]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem,Joint Int Res Lab, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontier Sci Res Base Optogenet Tech Cell, Shanghai 200237, Peoples R China

年份：2025

卷号：68

期号：1

起止页码：26

外文期刊名：SCIENCE CHINA-CHEMISTRY

收录：;EI(收录号：20243717020224);WOS:【SCI-EXPANDED(收录号:WOS:001308083300001)】；

基金：This work was supported by the National Key Research and Development Program of China (2023YFA1802000, 2021YFA0910000), the National Natural Science Foundation of China (22225805, 22308101, 32394001, 32121005), the Shanghai Science and Technology Innovation Action Plan (23J21901600), and the Innovation Program of Shanghai Municipal Education Commission, China Postdoctoral Science Foundation (2021M701199).

语种：英文

外文关键词：chemiluminescnece; sequence-activation; in-vivo; bioimaging; cyclic peroxide

摘要：Sequence-activated chemiluminescence (CL) technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution. The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest, thus enabling high-contrast signals under complex physiological conditions. This sequential activation could be triggered by enzymes, reactive oxygen species (ROS), biothiols or changes in the microenvironment. These unique sequence-activated CL probes bestow several advantages, including minimal background, deep-tissue penetration, and the ability of non-invasive or minimally invasive detection. The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics. In this mini-review, we focus on the latest developments in sequential CL probes, addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.