文献类型：期刊文献

英文题名：Tandem multimer expression and preparation of hypoglycemic peptide MC6 from Momordica charantia in Escherichia coli

作者：Wang, Fu-Jun[1,2]; Song, Huai-Lei[1]; Wang, Xiao-Meng[1,3]; Zhang, Wen-Ji[1]; Wang, Bi-Lian[1]; Zhao, Jian[1]; Hu, Zhi-Bi[1,2]

机构：[1] Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, China; [2] Institute of Chinese Materia Medica, Shanghai University of Chinese Traditional Medicine, Shanghai 201203, China; [3] School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

年份：2012

卷号：166

期号：3

起止页码：612

外文期刊名：Applied Biochemistry and Biotechnology

收录：EI(收录号：20121314896602)

语种：英文

外文关键词：Gene expression - Escherichia coli - Chlorine compounds - Peptides

摘要：Tandem repeat multimers of Momordica charantia (MC) peptide MC6 were designed and the recombinant plasmid containing 10 copies of MC6 gene was constructed to improve the expression level of MC6 in Escherichia coli. Under the selected conditions of cultivation and induction, the expression level of recombinant TrxA-MC610 protein was above 25% of total bacteria protein. This fusion protein was purified and cleaved with HCl (13%, w/v). Either the un-cleaved or cleaved recombinant proteins was analyzed pharmacological activity by alloxan-induced diabetic mice and only the cleaved products of the recombinant protein showed significant hypoglycemic effects. The study provides a convenient and economical method for the large-scale production of anti-diabetic medicines for pharmaceutical applications. ? Springer Science+Business Media, LLC 2011.