文献类型：期刊文献

中文题名：木瓜酶水解珍珠角壳蛋白

英文题名：A Study on Hydrolysis of Protein in Pearls by Papain

作者：金凤明[1];李桂贞[2];孙晓娟[1]

机构：[1]江苏石油化工学院化学工程系,江苏常州213016;[2]华东理工大学分析测试中心,上海200237

年份：2000

卷号：12

期号：3

起止页码：33

中文期刊名：江苏石油化工学院学报

外文期刊名：Journal of Jiangsu Institute of Petrochemical Technology

语种：中文

中文关键词：珍珠;蛋白质;木瓜酶;水解;角壳蛋白;淡水养殖

外文关键词：pearls;protein;papain;hydrolysis

摘要：用异硫氰酸苯酯柱前衍生高效液相色谱法测定了淡水养殖珍珠中角壳蛋白的氨基酸组成 ,对影响木瓜酶水解珍珠角壳蛋白的主要因素如木瓜酶用量、蛋白质质量浓度、反应时间、酸度和温度等进行了研究和优化 。
The contents of amino acids in protein of pearls were determined by reverse-phase high-performance liquid chromatography after precolumn derivatization with phenylisothiocyanate. The effect of such factors as dosage of papain, concentration of protein, time of reaction, acidity, temperature on hydrolytic ratio of protein in pearls by papain was studied. A simple and no-pollution technique for hydrolyzing protein in pearls by papain to obtain amino acids and peptides was developed in order to provide a basis for the effective use of pearl resources in our country.