文献类型：期刊文献

中文题名：柠檬酸钠对肌苷发酵过程代谢流分布的调节

英文题名：Regulation of sodium citrate on metabolic flux distribution of inosine fermentation process

作者：陈双喜[1];黄明志[1];储炬[1];庄英萍[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：2005

卷号：56

期号：9

起止页码：1731

中文期刊名：化工学报

外文期刊名：CIESC Journal

收录：CSTPCD;;EI(收录号：2005439438917);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市曙光计划资助项目(01SG28);上海市重点学科建设项目~~

语种：中文

中文关键词：枯草杆菌;肌苷;碳源溢流;代谢流调节

外文关键词：Bacillus subtilis; inosine; carbon overflow; flux regulation

摘要：基于物料的质量守恒定律和中间代谢物的拟稳态假设,定量研究了柠檬酸钠对肌苷发酵过程不同时段代谢流分布的影响.结果表明,添加的柠檬酸钠能够抑制存在于EMP途径和TCA循环之间的“碳源溢流”.在24h以后,EMP途径的代谢通量平均降低33%,HMP途径通量平均增加76%,肌苷合成途径通量平均增加77%,最终产苷由17.44g·L-1提高到24g·L-1,增幅达37.6%.
Metabolic flux analysis was used to understand macroscopical characteristics of fermentation process and cellular metabolic regulation. Based on the law of mass conservation and the assumption of pseudo-steady-state for the intermediates in the metabolic pathways, the effect of sodium citrate on the fluxes distribution of inosine fermentation process was quantitatively analyzed. It was revealed that carbon overflow between EMP pathways and TCA cycle could be restrained with the medium supplemented with sodium citrate. After 24 hours, the flux of EMP pathway decreased by 33% averagely, the flux of HMP pathway increased by 76% averagely, and the flux of inosine synthesis pathway increased by 77% on average. The final inosine yield was improved from 17.44 g·L^-1 to 24 g·L^-1 , by an increase of 37.6%.