文献类型：专利

英文题名：Constructing genetically engineered recombinant bacteria with surface expression of glutamic acid decarboxylase by linking surface primitive and glutamate decarboxylase gene to expression vector and introducing into host cell

作者：ZHAO L;FAN L;LI M;QIN Z;CHEN Q;QIU Y

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN106754610-A

申请日：2017-03-29

公开日：2017-05-31

语种：英文

收录：DERWENT

摘要：NOVELTY - Method of constructing genetically engineered recombinant bacteria having surface expression of glutamic acid decarboxylase involves linking surface primitive gene and glutamate decarboxylase encoding gene in series to the expression vector, constructing and screening recombinant expression vector, introducing the recombinant expression vector into the host cell to obtain the product. USE - The method is useful for constructing genetically engineered recombinant bacteria having surface expression of glutamic acid decarboxylase (claimed). ADVANTAGE - The method is simple, cost-effective, improves glutamic acid decarboxylase activity of cell, avoids permeation treatment, since the substrate need not enter the bacterial cell to contact recombinase to complete the transformation and enhances vitality of recombinant bacteria. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) genetically engineered recombinant bacteria having surface expression of glutamic acid decarboxylase, is constructed by performing the above-mentioned process; (2) method of expanding genetically engineered recombinant bacterial culture, involves (a) constructing genetically engineered recombinant bacteria, (b) inoculating the recombinant bacteria into the medium and culturing in a shaker to obtain seed liquid, (c) transferring the seed liquid to fresh culture medium and culturing in a shaker until the optical density (OD) OD 600 reaches 0.8-1, adding inducer to induce glutamate decarboxylase expression and collecting the recombinant bacteria; and (3) use of genetically engineered recombinant bacteria in preparation of gamma -aminobutyric acid by transforming glutamic acid into recombinant bacteria.