文献类型：期刊文献

英文题名：1,3-Propanediol production by a newly isolated strain, Clostridium perfringens GYL

作者：Guo, Yali[1,2];Dai, Lu[1,2];Xin, Bo[2];Tao, Fei[1,2,3];Tang, Hongzhi[2];Shen, Yaling[1];Xu, Ping[2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2017

卷号：233

起止页码：406

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20171203459570);WOS:【SCI-EXPANDED(收录号:WOS:000402475200050)】；

基金：This work was partially supported by the Chinese National Program for High Technology Research and Development (2014AA021206).

语种：英文

外文关键词：1,3-Propanediol; Anaerobic; Clostridium; Fermentation

摘要：1,3-Propanediol (1,3-PD), a valuable bulk industrial material, has attracted increasing interest in recent years. A novel strain GYL isolated from soil samples could efficiently convert glycerol to 1,3-PD anaerobically. The physiological and biochemical characteristics of strain GYL were determined, indicating that strain GYL is a member of Clostridium perfringens with the neighbor-joining method of 16S rRNA gene sequences. The fermentation properties of strain GYL were also investigated systematically, which showed that the strain has a fast growth speed and high tolerance to 200 g/L glycerol. Batch fermentation was carried out at a high glycerol concentration of 100 g/L, and strain GYL produced 36.7 g/L 1,3-PD. In fed-batch fermentation, strain GYL showed a maximum productivity of 2.0 g/(L.h), and produced 40.0 g/ L 1,3-PD, with a high yield of 0.68 mol 1,3-PD/mol glycerol. This study shows that the newly isolated strain GYL may have potential for 1,3-PD production from glycerol. (C) 2017 Elsevier Ltd. All rights reserved.