文献类型：期刊文献

英文题名：Chemically bonded maltose via click chemistry as stationary phase for HILIC

作者：Fu, Qing[1];Guo, Zhimou[1];Liang, Tu[2];Zhang, Xiuli[1];Xu, Qing[1];Liang, Xinmiao[1,2]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China

年份：2010

卷号：2

期号：3

起止页码：217

外文期刊名：ANALYTICAL METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000276805900003)】；

基金：Financial support from the Natural Science Foundation of China (Grant 20775079, 20805046) and Foundation for Distinguished Young Scholars (No 20825518) from the National Natural Sciences Foundation of China

语种：英文

摘要：A HILIC stationary phase was synthesized through bonding maltose on silica particles via click chemistry A set of representative polar compounds including sugars, ammo acids and small peptides were employed to evaluate chromatographic properties of Click Maltose The results illustrated the surface adsorption mechanism from the direct hydrogen bonding interaction between sugars and Click Maltose played an important role on retention behavior of sugars under HILIC mode The chromatographic behavior of anions on Click Maltose was consistent with HILIC/WAX mechanism, while the retention of cations was depended on electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) mechanism The Click Maltose stationary phase was successfully used in the separation of standard samples of polar compounds and complex samples of oligosaccharides from the natural product The Click Maltose also exhibited good reproducibility and stability, which were helpful to further application Our study confirmed the advantages and application potential of bonded oligosaccharides for HILIC separation