文献类型：期刊文献

中文题名：抑肽酶的亲和层析──Tosy1（对甲苯磺酰氯）在固定化酶上的应用

英文题名：Affinity Chromatography of Trasylol : The Application of Tosyl Agent inlmmobilized Enzyme

作者：冯万祥[1];施惠娟[1];诸镇祺[1]

机构：[1]华东理工大学生化研究所,江苏省张家港市啤酒厂

年份：1995

卷号：2

期号：4

起止页码：20

中文期刊名：药物生物技术

外文期刊名：Pharmaceutical Biotechnology

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：对甲苯磺酰氯;胰蛋白酶;4%琼脂糖;固定化酶;亲和层析;抑肽酶

外文关键词：Toasyl chloride;Trypsin;Sepharose 4B; Immobilized enzyme; Affinity chromatogra-phy; Trasylol

摘要：以４％琼脂糖为载体，对甲苯磺酰氯为活化剂，对胰蛋白酶的固定化条件及固定化酶的性质进行了研究。其固定化条件为ｐＨ７．４，温度１５℃，时间８ｈｒ，酶量２０ｍｇ／ｇ（ｗｅｔ－ｇｅｌ）。同时研究了温度、ｐＨ值、时间以及载体与酶的比例等因素对联蛋白酶固定化的影响。最后报道了该固定化酶的最适ｐＨ，热稳定性，ｐＨ贮存稳定性和操作稳定性。用这种固定化胰蛋白酶纯化抑肽酶，抑肽酶纯度提高５０倍，回收率在８５％～９５％。
his paper emphasized the study of immobilization of trypsin and the properties of immobillzed trypsinunder the condition of sepharose-4B as support and tosyl chloride as activating agent. vve found thatthe immobilizing condition was as follows:pH value., temp reaction time and trypsin were 7.4,15℃,8h. 20mg/lg(wet-gel)respectively. The effects of temp.,pH value,immobilizing time and the ratioof trypsin to sepharcoe-4B on trypsin immobilizition were studied. Trasylol was obtained from bovine lung extract, after affinity chromatography on immobilitedtrypsin. Its purity was raised 50 times, the rate of recovery was 90%～95%. We reported some findings of immobilized trypein:Optimum pH, heat steady, Ph store steady and oper-ation steady of immobilized trypsin as well. Some parameters of immobilized trypein were also deter-mined.