文献类型：期刊文献

英文题名：A genome-scale metabolic model of the effect of dissolved oxygen on 1,3-propanediol fermentation by Klebsiella pneumoniae

作者：Zhang, Yang[1];Yang, Menglei[1];Bao, Yangyang[1];Tao, Weihua[1];Tuo, Jinyou[1];Liu, Boya[1];Gan, Luxi[1];Fu, Shuilin[1];Gong, Heng[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：46

期号：9

起止页码：1319

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20232714359774);WOS:【SCI-EXPANDED(收录号:WOS:001020492900001)】；

基金：AcknowledgementsThis work was supported by the National Natural Science Foundation of China under Grant No. 31271862.

语种：英文

外文关键词：1; 3-Propanediol; Flux balance analysis; Genome-scale metabolic model; Klebsiella pneumoniae; Microaerobic culture

摘要：Although 1,3-propanediol (1,3-PD) is usually considered an anaerobic fermentation product from glycerol by Klebsiella pneumoniae, microaerobic conditions proved to be more conducive to 1,3-PD production. In this study, a genome-scale metabolic model (GSMM) specific to K. pneumoniae KG2, a high 1.3-PD producer, was constructed. The iZY1242 model contains 2090 reactions, 1242 genes and 1433 metabolites. The model was not only able to accurately characterise cell growth, but also accurately simulate the fed-batch 1,3-PD fermentation process. Flux balance analyses by iZY1242 was performed to dissect the mechanism of stimulated 1,3-PD production under microaerobic conditions, and the maximum yield of 1,3-PD on glycerol was 0.83 mol/mol under optimal microaerobic conditions. Combined with experimental data, the iZY1242 model is a useful tool for establishing the best conditions for microaeration fermentation to produce 1,3-PD from glycerol in K. pneumoniae.