文献类型：期刊文献

英文题名：Role of Trichoderma reesei Heterotrimeric G Protein Signaling in Growth and Cellulose Degradation

作者：Li, Ni[1,2];Yang, Xinyu[1];Cai, Wanchuan[1];Chen, Yumeng[1];Zhou, Zhihua[2,3];Wang, Wei[1,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Synthet Biol, 300 FengLin Rd, Shanghai 200032, Peoples R China;[3]Nanjing Agr Univ, Acad Adv Interdisciplinary Studies, Nanjing 210014, Peoples R China;[4]Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Engn Biol Low Carbon Mfg, Tianjin 300308, Peoples R China

年份：2026

卷号：74

期号：20

起止页码：15596

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20262320844479);WOS:【SCI-EXPANDED(收录号:WOS:001769412000001)】；

基金：This work was supported by the National Natural Science Foundation of China [no. 32370093 to W.W.], National Key Research and Development Program of China [grant no. 2022YFA0912300 to W.W.], Natural Science Foundation of Shanghai [no. 23ZR1414800 to Y.C.], and Shanghai Postdoctoral Excellence Program [2023642 to L.N.].

语种：英文

外文关键词：G proteins; Trichoderma reesei; ACY1-cAMP-PKA pathway; cellulase production

摘要：Trichoderma reesei decomposes renewable cellulose into soluble sugars by secreting cellulase and hemicellulase. Heterotrimeric G protein signaling regulates cellulase production. Here, we assessed the effects of G protein subunit deletion on cellulase production in T. reesei. The results revealed that G proteins bidirectionally regulate cellulase production, activation, and repression; GNA1, GNA3, TGB1, and GBG1 downregulate cellulase synthesis, whereas GNA2 upregulates it. To understand the role of the ACY1-cAMP-PKA pathway and G protein signaling in cellulase expression, we overexpressed/knocked out acy1 in G protein subunit-knockout strains. GNA1 orchestrates the G protein signaling pathway, with its core function being the transmission of glucose repression signals. GNA3 serves as a complement to GNA1. GNA2 is the only G alpha subunit that transduces cellulose-induced signals. The ACY1-cAMP-PKA pathway can act as an activator, a repressor, or an inactive regulator in G protein signal transduction. Our study highlights avenues for constructing cellulase-hyperproducing strains.