文献类型：期刊文献

英文题名：Chemical control over the conversion between bicyclic and polycyclic terpenes by fungal bifunctional terpene synthases

作者：Wang, Xinye[1];Wang, Zhixin[1];Zhu, Guoliang[1];Jiang, Lan[1];Zhang, Weiyan[1];Huang, Yiyi[1];Cong, Zhanren[1];Zhao, Yi-Lei[2,3];Xu, Jian-He[1];Hsiang, Tom[4];Zhang, Lixin[1];Chen, Qi[1];Liu, Xueting[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, State Key Lab Microbial Metab,MOE LSB, Shanghai 200240, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, MOE LSC, Shanghai 200240, Peoples R China;[4]Univ Guelph, Sch Environm Sci, 50 Stone Rd East, Guelph, ON N1G 2W1, Canada

年份：2022

卷号：58

期号：68

起止页码：9476

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20223212553684);WOS:【SCI-EXPANDED(收录号:WOS:000834535000001)】；

基金：This work was supported by the National Key Research and Development Program of China (2019YFA0906201 and 2020YFA090032), the National Natural Science Foundation of China (31720103901, 21977029, 21907031, 81903529 and 21877038), and the China Postdoctoral Science Foundation (2019M661403). We sincerely thank Prof. Yi Tang at the University of California for the gift of plasmid pXW55. We are grateful to Prof. Pinghhua Liu at Boston University and Prof. Hideaki Oikawa at Hokkaido University for their valuable suggestions.

语种：英文

外文关键词：Catalysis - Terpenes

摘要：Fungal bifunctional terpene synthases (BFTSs) reportedly associate with a series of new skeletons of di/sesterterpenes. However, the molecular mechanisms underlying the variabilities in the ring system of BFTS-catalyzed products are not well understood. In this study, we identified a key site, S89/L89, that controls the conversion between bicyclic and polycyclic terpene skeletons catalyzed by two BFTSs, BsPS and FoFS. Our analysis revealed that a mutation on site 89 in the BFTSs alters the carbocation transportation pathway and redirects the competing reactions for previously unreported terpenes.