文献类型：期刊文献

中文题名：林可霉素生物合成缺陷的同源重组菌鉴定

英文题名：Identification of Homologous Recombination of Lincomycin Biosynthesis Defective Strains

作者：沈琼[1];吴海珍[1];唐雅珺[1];张惠展[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2003

卷号：34

期号：4

起止页码：164

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：林可霉素;生物合成;同源重组菌;效价;鉴定

外文关键词：biosynthesis of lincomycin; homologous recombination; potency of lincomycin

摘要：以 S.lincolnensis B48为对照 ,分别测定两个染色体上含 lmb I基因灭活拷贝的菌株 YY1(同源交换 )、YY2 (同源整合 )的抗菌效价 ,其中 YY1在其整个生物周期中均无明显抗菌效价 ,而 YY2的效价与 B48相比也明显偏低。进一步通过 PCR反应验证这两株基因工程菌 lmb I基因灭活情形 ,结果发现 YY 1染色体上 lmb I基因的两个拷贝均因同源交换而被灭活 ,而 YY2可能是因单拷贝发生同源整合而仅被部分灭活。
Strain YY1 and YY2 were two genetic recombinant strains derived from S.lincolnensis B48 by homologous exchange and homologous integration, respectively. Compared with B48, the potency of lincomycin of YY2 was much lower, while that of YY1 turned to be zero. The genetic recombination was further identified by PCR and the result showed that two copies of lmbI genes in YY1 were inactivated by homologous exchange, while probably only one copy in YY2 was inactivated by homologous integration and the other remained intact.