文献类型：期刊文献

中文题名：增加复制起始区对基因表达的影响

英文题名：Improvement of Expression Level by Increasing Number of Replication Origins in Piasmid

作者：王二力[1];姚国伟[1];张晓晖[1];曹雪颖[1]

机构：[1]华东理工大学基因工程研究室

年份：1995

卷号：25

期号：2

起止页码：1

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：复制起始区;基因表达

外文关键词：Replication origin, Gene expression

摘要：我们在质粒puB110的基础上组建了pDR质粒,它们具有双复制起始区而只有一个抗卡那霉素基因。携带了这些质粒的宿主细胞对卡那霉素的抗性明显高于亲本株〔B.subtilis 150 (puB110)〕。经限制性酶切图谱分析新获得的转化株具有二个复制起始区及一个Km^r基因。说明增加复制起始区是提高重组子表达能力的途径之一。
A plasmid pDR carrying two replication regions and only one resistant to kanamycin gene from the plasmid pUB 110 was constructed. The host presented a higher level of kanamycin gene expression than that of the original strain B. subtilis 150(pUB110). The restrictive map of plasmid pDR was determined and its characterization of resistance to Km showed that the improvement of the expression level could also be achieved by increasing the number of replication origins.