文献类型：期刊文献

英文题名：Whole-cell one-pot biosynthesis of dodecanedioic acid from renewable linoleic acid

作者：Qi, Yi-Ke[1,2];Pan, Jiang[1];Zhang, Zhi-Jun[1];Xu, Jian-He[1]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Hebei Univ Sci & Technol, Coll Food Sci & Biol, 26 Yuxiang St, Shijiazhuang 050018, Peoples R China

年份：2024

卷号：11

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20242216172058);WOS:【SCI-EXPANDED(收录号:WOS:001230267700001)】；

基金：We thank the research and development platform provided by State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Bio manufacturing, East China University of Science and Technology.

语种：英文

外文关键词：Linoleic acid; Dodecanedioic acid; Escherichia coli; Whole-cell biosynthesis; Multi-enzymatic cascade

摘要：Background Dodecanedioic acid (DDA), a typical medium-chain dicarboxylic fatty acid with widespread applications, has a great synthetic value and a huge industrial market demand. Currently, a sustainable, eco-friendly and efficient process is desired for dodecanedioic acid production. Results Herein, a multi-enzymatic cascade was designed and constructed for the production of DDA from linoleic acid based on the lipoxygenase pathway in plants. The cascade is composed of lipoxygenase, hydroperoxide lyase, aldehyde dehydrogenase, and unidentified double-bond reductase in E. coli for the main cascade reactions, as well as NADH oxidase for cofactor recycling. The four component enzymes involved in the cascade were co-expressed in E. coli, together with the endogenous double-bond reductase of E. coli. After optimizing the reaction conditions of the rate-limiting step, 43.8 g L- 1 d(- 1) of DDA was obtained by a whole-cell one-pot process starting from renewable linoleic acid. Conclusions Through engineering of the reaction system and co-expressing the component enzymes, a sustainable and eco-friendly DDA biosynthesis route was set up in E. coli, which afforded the highest space time yield for DDA production among the current artificial multi-enzymatic routes derived from the LOX-pathway, and the productivity achieved here ranks the second highest among the current research progress in DDA biosynthesis.