文献类型：期刊文献

英文题名：Separation of carbohydrates using hydrophilic interaction liquid chromatography

作者：Fu, Qing[1];Liang, Tu[1];Li, Zhenyu[1];Xu, Xiaoyong[1];Ke, Yanxiong[1];Jin, Yu[1];Liang, Xinmiao[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China

年份：2013

卷号：379

起止页码：13

外文期刊名：CARBOHYDRATE RESEARCH

收录：;EI(收录号：20134116838230);WOS:【SCI-EXPANDED(收录号:WOS:000324301300003)】；

基金：The authors gratefully acknowledge Professor Yuguang Du (Natural Products and Glyco-biotechnology, DICP) for the samples of oligosaccharide. This work was supported by 'the Fundamental Research Funds for the Central Universities' (WY1214070) and National Natural Science Funds (21005028).

语种：英文

外文关键词：Carbohydrates; Hydrophilic interaction liquid chromatography; Stationary phase; Selectivity; Chromatographic characterization

摘要：A strategy was developed to rapidly evaluate chromatographic properties of hydrophilic interaction chromatography (HILIC) columns for separating carbohydrates. Seven HILIC columns (Silica, Diol, TSK Amide-80, XAmide, Click Maltose, Click beta-CD, and Click TE-Cys columns) were evaluated by using three monosaccharide and seven disaccharides as probes. The influence of column temperature on the peak shape and tautomerization of carbohydrates, as well as column selectivity were investigated. The influence of surface charge property on the retention was also studied by using glucose, glucuronic acid, and glucosamine, which indicated that buffer salt concentration and pH value in mobile phase was necessary to control the ionic interactions between ionic carbohydrates and HILIC columns. According to evaluation results, the XAmide column was selected as an example to establish experimental schemes for separation of complex mixtures of oligosaccharide. (C) 2013 Elsevier Ltd. All rights reserved.