文献类型：期刊文献

英文题名：A dextran-bonded stationary phase for saccharide separation

作者：Sheng, Qianying[1];Su, Xindai[3];Li, Xiuling[2];Ke, Yanxiong[1];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China;[3]Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China

年份：2014

卷号：1345

起止页码：57

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20142017715767);WOS:【SCI-EXPANDED(收录号:WOS:000336709300008)】；

基金：This work was supported by National High Technology Research and Development Program of China (863 Program, 2012AA020203) and National Natural Science of China (Grant No. 21135005 and No. 21375038).

语种：英文

外文关键词：Dextran; Oligosaccharide; Glycopeptides; HILIC; MS

摘要：A-saccharide-based stationary phase for hydrophilic interaction liquid chromatography (HILIC) is presented. The method uses carbonyl di-imidazole (CDI) as a cross-linker for dextran in aqueous solution. Different from the traditional immobilized saccharide stationary phase, this method was using a simple way to bond the high molecular of polysaccharide immobilized on the silica gel. The new method avoided the time-consuming process but had a very considerable loading result. With silica-based surface bonded, it will be possible to develop well-defined surface modifications that promote the hydrophilic with dextran and high mechanical strength with silica. For all tested compounds, including polar compounds and carbohydrates, this dextran-bonded stationary phase performed well in terms of separation efficiency and column stability, and the retention mechanism matched that of typical HILIC retention. Moreover, good selectivity was achieved in the separation of oligosaccharides and glycopeptides. (C) 2014 Elsevier B.V. All rights reserved.