文献类型：期刊文献

英文题名：MBG-Modified β-TCP Scaffold Promotes Mesenchymal Stem Cells Adhesion and Osteogenic Differentiation via a FAK/MAPK Signaling Pathway

作者：Liu, Yutong[1,3];Ma, Yifan[1,3];Zhang, Jing[1,3];Xie, Qing[4];Wang, Zi[4];Yu, Shuang[3];Yuan, Yuan[3];Liu, Changsheng[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Biomat, Minist Educ, Shanghai 200237, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Ophthalmol, Shanghai 200011, Peoples R China

年份：2017

卷号：9

期号：36

起止页码：30283

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20173804181532);WOS:【SCI-EXPANDED(收录号:WOS:000411043600009)】；

基金：The authors express their gratitude for financial support from the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the National Natural Science Foundation of China (Nos. 31330028 and 31470924), and the 111 Project (B14018).

语种：英文

外文关键词：MBG-modified beta-TCP scaffolds; mechanical strength; microstructure; cell affinity and osteogenic capacity; FAK/MAPK signaling pathway

摘要：The beta-TCP scaffold has been widely used as a bone graft substitute, but the traditional PMMA molding method induced undesirable mechanical strength and poor interconnectivity still have not been addressed until now. In this study, a MBG-based PU foam templating method was developed to fabricate beta-TCP scaffolds with desirable microtopography. The MBG gel, as both binder and modifier, prepared by a modified sol gel method with controlled viscosity is incorporated with beta-TCP powder and thereafter is impregnated into PU foam. The resultant hybrid scaffolds exhibited interconnected macropores (200 - 500 mu m) and distinctive micropores (0.2-1.5 mu m), especially for the TCP/25MBG (with 25 wt % content MBG). As expected, the compression strength of beta-TCP/MBG composite scaffolds was enhanced with increasing MBG content, and TCP/50MBG (with SO wt % content MBG) exhibited almost 100-fold enhancement compared to the pure beta-TCP. Intriguingly, the cell affinity and osteogenic capacity of rBMSCs were also dramatically improved the best on TCP/25MBG. Further investigation found that the subtle, grainy-like microtopography, not the chemical composition, of the TCP/25MBG favored the adsorption of Fn and expression of integrin (alpha 5/beta 1 and further facilitated FA formation and the expression of p-FAK, following activation of the MAPK/ERK signaling pathway and ultimately upregulated expression of osteogenic genes. Further in vivo experiments confirmed the promoted osteogenesis of TCP/25MBG in vivo. The results suggest that such a novel MBG-based PU foam templating method offers new guidance to construct hierarchically porous scaffolds, and the prepared MBG-modified beta-TCP scaffold will have great potential for future use in bone tissue regeneration.