文献类型：期刊文献

英文题名：An ESIPT Probe for the Ratiometric Imaging of Peroxynitrite Facilitated by Binding to Aβ-Aggregates

作者：Sedgwick, Adam C.[1,4];Dou, Wei-Tao[2];Jiao, Jin-Biao[2];Wu, Luling[1];Williams, George T.[1];Jenkins, A. Toby A.[1];Bull, Steven D.[1];Sessler, Jonathan L.[4];He, Xiao-Peng[2];James, Tony D.[1,3]

机构：[1]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Sophia Univ, Fac Sci & Technol, Dept Mat & Life Sci, Chiyoda Ku, 7-1 Kioi Cho, Tokyo 1028554, Japan;[4]Univ Texas Austin, Dept Chem, 105 East 24th St A5300, Austin, TX 78712 USA

年份：2018

卷号：140

期号：43

起止页码：14267

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20184305982191);WOS:【SCI-EXPANDED(收录号:WOS:000449239700037)】；

基金：We thank the EPSRC and the University of Bath for funding. A.C.S. and A.T.A.J. thank the EPSRC for funding (EP/R003939/1). T.D.J. wishes to thank the Royal Society for a Wolfson Research Merit Award and Sophia University for a visiting professorship. NMR characterisation facilities were provided through the Chemical Characterisation and Analysis Facility (CCAF) at the University of Bath (www.bath.ac.uk/ccaf). The EPSRC U.K. National Mass Spectrometry Facility at Swansea University is thanked for analyses. Hai-Hao Han and Jing-Jing Zhang are thanked for biological experiments. X.-P.H. thanks the National Natural Science Foundation of China (21788102, 21722801, and 21572058), the Programme of Introducing Talents of Discipline to Universities (B16017), and the Shanghai Rising-Star Program (16QA1401400) for support. J.L.S. thanks The Robert A. Welch Foundation (F-0018). He Tian is warmly thanked for his helpful advice. We would like to thank Lauren Gwynne for proof reading the manuscript.

语种：英文

外文关键词：Proteins - Excited states - Aggregates

摘要：A series of 3-hydroxyflavone (3-HF) ESIPT (excited-state intramolecular proton transfer) boronate-based fluorescent probes have been developed for the detection of peroxynitrite (ONOO-). The dyes are environmentally sensitive, and each probe exhibited a ratiometric response toward ONOO- in a micellar environment. The probes were used to image different aggregation states of amyloid-beta (A beta) in the presence of ONOO-. The 3-HF-OMe probe was found to produce a ratiometric response toward ONOO- when bound to A beta aggregates, resulting in a novel host-guest ensemble, which adds insight into the development of other ESIPT-based probes for the simultaneous sensing of fibrous proteins/peptides and environmental ROS/RNS.