文献类型：期刊文献

英文题名：The antimicrobial effect of calcium-doped titanium is activated by fibrinogen adsorption

作者：Cao, Huiliang[1,2];Dauben, Thomas J.[2];Helbing, Christian[2];Jia, Zhichao[1];Zhang, Yuechao[1];Huang, Moran[3];Mueller, Lenka[4];Gu, Song[2];Zhang, Xiaoyuan[2];Qin, Hui[5];Martin, Karin[6];Bossert, Joerg[2];Jandt, Klaus D.[2,7]

机构：[1]East China Univ Sci & Technol, Shanghai Engn Res Ctr Hierarch Nanomat, Sch Mat Sci & Engn,Key Lab Ultrafine Mat,Minist E, Lab Low Dimens Mat Chem,Interfacial Electrochem &, Shanghai 200237, Peoples R China;[2]Friedrich Schiller Univ Jena, Otto Schott Inst Mat Res, Chair Mat Sci, D-07743 Jena, Germany;[3]Univ Sci & Technol China, Dept Orthoped, Affiliated Hosp 1, Hefei 230001, Peoples R China;[4]Friedrich Schiller Univ Jena, Otto Schott Inst Mat Res, Colloids Surfaces & Interfaces, D-07743 Jena, Germany;[5]Shanghai Jiaotong Univ Affiliated Peoples Hosp 6, Dept Orthopaed, Shanghai 200233, Peoples R China;[6]Leibniz Inst Nat Prod Res & Infect Biol, Hans Knoll Inst, Bio Pilot Plant, D-07745 Jena, Germany;[7]Friedrich Schiller Univ Jena, Jena Ctr Soft Matter JCSM, D-07743 Jena, Germany

年份：2022

卷号：9

期号：7

起止页码：1962

外文期刊名：MATERIALS HORIZONS

收录：;EI(收录号：20223112450489);WOS:【SCI-EXPANDED(收录号:WOS:000797033500001)】；

基金：This work was jointly supported by grants from the National Natural Science Foundation of China (31870945 and 31771022), the Natural Science Foundation of Shanghai (21ZR1415700), and the College Student Innovation and Entrepreneurship Training Program (X202110251166 and S202110251049). Dr Cao thanks the Alexander von Humboldt Foundation for a research fellowship award. The authors would like to thank Dipl.-Ing. (FH) Heidrun Garlipp for SEM imaging, Dipl.-Ing. (FH) Ralf Wagner for the XPS analysis of the samples, and Ms Ines Thiele for the preparation of bacterial samples for SEM.

语种：英文

外文关键词：Adsorption - Amides - Bacteria - Binding energy - Fourier transform infrared spectroscopy - Mammals - Proteins - Titanium - Toxic materials - X ray photoelectron spectroscopy

摘要：Directly targeting bacterial cells is the present paradigm for designing antimicrobial biomaterial surfaces and minimizing device-associated infections (DAIs); however, such pathways may create problems in tissue integration because materials that are toxic to bacteria can also be harmful to mammalian cells. Herein, we report an unexpected antimicrobial effect of calcium-doped titanium, which itself has no apparent killing effect on the growth of pathogenic bacteria (Pseudomonas aeruginosa, Pa, ATCC 27853) while presenting strong inhibition efficiency on bacterial colonization after fibrinogen adsorption onto the material. Fine X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy analyses reported calcium-dependent shifts of the binding energy in nitrogen and oxygen involved groups and wavenumbers in the amide I and II bands of the adsorbent fibrinogen, demonstrating that locally delivered calcium can react with the carboxy-terminal regions of the A alpha chains and influence their interaction with the N-termini of the B beta chains in fibrinogen. These reactions facilitate the exposure of the antimicrobial motifs of the protein, indicating the reason for the surprising antimicrobial efficacy of calcium-doped titanium. Since protein adsorption is an immediate intrinsic step during the implantation surgery, this finding may shift the present paradigm on the design of implantable antibacterial biomaterial surfaces.