文献类型：期刊文献

英文题名：Characterization of two TonB systems in marine fish pathogen Vibrio alginolyticus:: their roles in iron utilization and virulence

作者：Wang, Qiyao[1];Liu, Qin[1];Cao, Xiaodan[1];Yang, Minjun[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：190

期号：5

起止页码：595

外文期刊名：ARCHIVES OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000259934500009)】；

基金：This work was supported by grants from the Chinese National Natural Science Foundation (No. U0633004) and Shanghai Leading Academic Discipline Project (No. B505).

语种：英文

外文关键词：iron transportation; TonB system; Vibrio alginolyticus; virulence

摘要：In Gram-negative bacteria, the TonB/ExbB/ExbD complex is required to energize the specific high-affinity receptors mediating iron uptake processes. In fish pathogen Vibrio alginolyticus MVP01, which exhibited capacities to scavenge iron sources with various specific iron uptake systems, we identified and characterized two sets of TonB systems. In V. alginolyticus, TonB1 and TonB2 systems were arrayed as TonB1-ExbB1-ExbD1 and ExbB2-ExbD2-TonB2, respectively. The transcription of the TonB systems was regulated by the iron concentration in culture medium. While TonB1 system specifically contributed to the hemin and hemoglobin utilization in V. alginolyticus MVP01, both of the TonB systems showed abilities to support the utilization of iron sources from ferrichrome and vibrioferrin, the endogenous siderophore of V. alginolyticus. In addition, when mutants of the TonB systems were inoculated intraperitoneally into the fish Brachydanio rerio, marked attenuation in virulence of V. alginolyticus was observed, indicating that both of the TonB systems are essential for the virulence of V. alginolyticus.